| HPLC法测定温毒清浸膏中栀子苷的含量 |
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| 引用本文： | 汤法银,樊克锋,王坤丽.HPLC法测定温毒清浸膏中栀子苷的含量[J].中兽医医药杂志,2012,31(4):51-53. |
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| 作者姓名： | 汤法银 樊克锋 王坤丽 |
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| 作者单位： | 郑州牧业工程高等专科学校药物工程系,河南郑州,450008 |
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| 基金项目： | 河南省教育厅自然科学研究项目（2010A230013） |
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| 摘 要： | 采用HPLC方法测定温毒清浸膏中栀子苷的含量。流动相:乙腈-水(15∶85);流速:1 mL/min;检测波长:238 nm;色谱柱:Diamonsi L C18(5μm,200 mm×4.6 mm);柱温:25℃;理论塔板数不低于1 500。结果显示,栀子苷进样量在0.060-0.600μg范围内有良好的线性关系,平均回收率99.32%,RSD为1.26%。表明该方法准确可靠,重现性好,可作为温毒清浸膏的质量控制方法。
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| 关 键 词： | 温毒清浸膏 栀子苷 含量测定 HPLC |
Content determination of geniposide in Wenduqing extractum by HPLC |
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| TANG Fa-yin,FAN Ke-feng,WANG Kun-li.Content determination of geniposide in Wenduqing extractum by HPLC[J].Journal of Traditional Chinese Veterinary Medicine,2012,31(4):51-53. |
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| Authors: | TANG Fa-yin FAN Ke-feng WANG Kun-li |
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| Institution: | (Zhengzhou College of Animal Husbandry Engineering, Zhengzhou Henan450011, China) |
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| Abstract: | Objective： To determine the content of geniposide in Wenduqing extractum by HPLC. Methods： The mobile phase was acetonitrile and water （15：85）with the flow rate of 1 mL/min at 238 nm, the chromatographic column was Diamonsi L Cts （5 μm,200 min×4.6 mm） at 25℃, and theoretical plate number was no less than 1 500. Results： There was a good linearity for geniposide in Wenduqing extractum in the range of 0.060-0.600μg with average recovery rate of 99.32% and RSD of 1.26%. Conclusion： HPLC was accuracy and repeatability. suitable to use to control the quality of Wenduqing extractum for its accuracy and repeatability, |
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| Keywords: | Wernduqing extractum geniposide content determination HPLC |
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