| 高效液相色谱-蒸发光散射法测定芪参口服液中黄芪甲苷的含量 |
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| 引用本文： | 王建舫,胡格,张涛,高会军,宣颖超,李佳,穆祥.高效液相色谱-蒸发光散射法测定芪参口服液中黄芪甲苷的含量[J].中兽医医药杂志,2012,31(5):11-14. |
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| 作者姓名： | 王建舫 胡格 张涛 高会军 宣颖超 李佳 穆祥 |
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| 作者单位： | 1. 北京农学院兽医学中医药北京市重点实验室,北京,102206
2. 石家庄华牧牧业有限责任公司兽药分公司 |
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| 基金项目： | 国家“十二五”规划“国家蛋鸡产业技术体系”（CARS-41-S05） |
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| 摘 要： | 本试验旨在建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定芪参口服液中黄芪甲苷含量的方法。采用YMC-Pack ODS-A色谱柱(5μm,150 mm×4.6 mm),以乙腈-水(35∶65)为流动相,流速1.0 mL/min,柱温30℃,飘移管温度65℃,氮气压力30 psi。结果表明,黄芪甲苷对照品在0.60-6.00μg范围内线性关系良好(r=0.999 6),平均加样回收率为97.88%(n=6),峰面积的相对标准偏差(RSD)为3.20%。该方法灵敏、准确、重复性好,可作为芪参口服液的质量控制方法。
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| 关 键 词： | 芪参口服液 黄芪甲苷 高效液相色谱-蒸发光散射检测器 |
Content determination of Astragoloside IV in Qishen oral solution by HPLC-ELSD |
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| WANG Jian-fang,HU Ge,ZHANG Tao,XUAN Ying-ehao,LI Jia,MU Xiang.Content determination of Astragoloside IV in Qishen oral solution by HPLC-ELSD[J].Journal of Traditional Chinese Veterinary Medicine,2012,31(5):11-14. |
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| Authors: | WANG Jian-fang HU Ge ZHANG Tao XUAN Ying-ehao LI Jia MU Xiang |
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| Institution: | (Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China) |
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| Abstract: | This experiment was conducted to establish a method for determining the content of Astragoloside Ⅳ in Qishen oral solution by HPLC-ELSD.The chromatographic separation was carried out on YMC-Pack ODS-A column（5 μm,150 mm×4.6 mm）.The mobile phase composed of methyl cyanide-water（35∶65）was used at the flow rate of 1 mL/min with the column temperature of 30 ℃.The drift tube temperature of the evaporative light-scattering detector was 65 ℃ and N 2 pressure was 30 psi.Astragoloside Ⅳ showed good linearity（r=0.999 6） in the range of 0.60-6.00 μg,and the average recovery was 97.88% with RSD of 3.20%.The method is sensitive,accurate and repeatable,and can be used for the quality control of Qishen oral solution. |
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| Keywords: | Qisben oral solution Astragoloside 1V HPLC-ELSD |
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