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| 直、支链木薯淀粉的分离纯化及检测 | |
| 引用本文： | 王彦超,郝再彬,李子院,李海云,杨丹.直、支链木薯淀粉的分离纯化及检测[J].东北农业大学学报,2009,40(3). |
| 作者姓名： | 王彦超 郝再彬 李子院 李海云 杨丹 |
| 作者单位： | 1. 东北农业大学生命科学学院,哈尔滨,150030 2. 东北农业大学生命科学学院,哈尔滨,150030;桂林工学院,广西,桂林,541004 3. 桂林工学院,广西,桂林,541004 |
| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
| 摘 要： | 以自制的木薯淀粉为原料,采用正丁醇反复结晶法制备直链淀粉和支链淀粉样品,并对原淀粉和直、支链淀粉进行定性、定量测定。结果表明,利用酶解法对淀粉大分子进行酶解后,通过测定还原糖的含量可实现对淀粉各组分的定量测定,为木薯淀粉各组分的进一步分级与检测研究提供理论基础。 |
| 关 键 词： | 木薯淀粉 直链 支链 酶解 麦芽糖 |
Separation and determination of amylose and amylopectin from cassava starch |
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| WANG Yanchao,HAO Zaibin,LI Ziyuan,LI Haiyun,YANG Dan.Separation and determination of amylose and amylopectin from cassava starch[J].Journal of Northeast Agricultural University,2009,40(3). | |
| Authors: | WANG Yanchao HAO Zaibin LI Ziyuan LI Haiyun YANG Dan |
| Institution: | 1. College of Life Sciences;Northeast Agricultural University;Harbin 150030;China;2. Guilin University of Technology;Guilin Guangxi 541004;China |
| Abstract: | Amylose and amylopectin were separated from cassava starch by Butanol recrystallization method, qualitative and quantitative determination of amylose and amylopectin were studied, too. The results showed that the content of amylose and amylopectin in cassava starch could be identified quantitatively by determining the content of reducing sugar after enzymatic hydrolysis. It provides theoretical basis for grading and determining of cassava starch components. |
| Keywords: | cassava starch amylose amylopectin enzymatic hydrolysis maltose |
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