| 猪伪狂犬病毒YZ株gE全基因的克隆与序列分析 |
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| 引用本文： | 朱前磊,方先珍,程果,毕亚楠,乔涵,付朋飞,王淑娟,陈红英.猪伪狂犬病毒YZ株gE全基因的克隆与序列分析[J].安徽农学通报,2015(7):127-129. |
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| 作者姓名： | 朱前磊 方先珍 程果 毕亚楠 乔涵 付朋飞 王淑娟 陈红英 |
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| 作者单位： | 河南省动物疫病预防控制中心,河南郑州,450008;河南农业大学牧医工程学院,河南郑州,450002 |
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| 基金项目： | 河南省重大科技专项(111100110300)；河南省产学研项目(132107000002)。 |
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| 摘 要： | 为了解猪伪狂犬病毒(PRV)g E基因的遗传变异特性,根据Gen Bank已发表的PRV g E全基因序列,设计并合成1对特异性引物,对PRV YZ株进行了g E全基因的扩增、克隆及测序,并与其他参考毒株g E基因进行比较序列分析。测序结果表明,YZ株g E全基因序列由1 862bp组成,与Gen Bank已发表的13株PRV g E参考株序列同源性介于97.9%~99.9%。进化树分析表明,YZ株与目前国内流行的毒株在同一进化分支内,与国外分离株有一定差异。
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| 关 键 词： | 伪狂犬病毒 gE基因 克隆 序列分析 |
Cloning and Sequence Analysis of the Full-length gE Gene of Pseudorabies Virus YZ Strain |
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| Abstract: | In order to investigate the genetic mutation features of PRV gE,one pair of primers was de?signed and synthesized to amplify the full-length gE gene of PRV YZ strain according to the genomic se?quence of PRV gE published in GenBank.PRV gE gene was amplified by PCR.The purified PCR products were cloned into pMD18–T vector and sequenced.The sequencing results showed that the full-length gE gene of PRV YZ consisted of 1 862 nucleotides,the homologies were 97.9%~99.9% when compared with gE sequences of 13 PRV published in GenBank.Phylogenetic analysis showed that PRV YZ isolate had a closer relationship with other domestic isolates than foreign reference strains. |
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| Keywords: | Pseudorabies virus gE gene Cloning Sequence analysis |
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