| 农大5号钙果叶片芽诱导及其植株再生研究 |
| |
| 引用本文： | 韩向峰,李志芳,曹 琴,张海平,程小爱.农大5号钙果叶片芽诱导及其植株再生研究[J].广东农业科学,2018,45(8):37-42. |
| |
| 作者姓名： | 韩向峰 李志芳 曹 琴 张海平 程小爱 |
| |
| 作者单位： | 佛山市农业科学研究所;山西农业大学园艺学院;定襄县国有林场;太谷县农业委员会 |
| |
| 摘 要： | 采集定植于温室中苗木上的叶片和组培瓶中农大5号钙果分化苗、壮苗叶片,进行离体叶片再生芽诱导及其植株再生体系研究。结果表明,温室叶片诱导不定芽的最佳培养基为1/2MS+IAA 0.1 mg/L+NAA0.05 mg/L+6-BA 0.8 mg/L,培养基中全部元素减半;组培分化苗叶片和壮苗叶片诱导不定芽的最佳培养基为1/2MS+IAA 0.05 mg/L+NAA 0.1 mg/L+6-BA 0.8 mg/L,培养基中大量元素减半;辅助因子以培养基中添加AgNO_3 0.2 mg/L、全光照、叶片反放和带叶柄的叶基部等措施配合取得了最高的芽诱导率。
|
| 关 键 词： | 欧李 农大5号 叶片 不定芽 再生 |
Study on bud induction and regeneration from
leaves of Nongda No.5 in Chinese dwarf cherry |
| |
| Abstract: | In this study, leaves of Chinese dwarf cherry variety Nongda No.5 were collected from greenhouse
cultivated seedlings and tissue cultured seedlings respectively. Bud induction and plant regeneration were studied.
Theoptimum medium for adventitious buds induction of leaves from greenhouse was 1/2MS+IAA 0.1 mg/L+NAA
0.05 mg/L+6-BA 0.8 mg/L, in which all the nutrients were halved.The best medium for adventitious buds induction
of leaves from tissue cultured seedlings was1/2MS+IAA 0.05 mg/L+NAA 0.1 mg/L+6-BA 0.8 mg/L, in which
macro-elements were halved. Moreover, auxiliary factor studies showed that the highest bud induction rate was
achieved with the combination of adding 0.2 mg/L AgNO3 into the medium, full light exposure, turning leaves
upside down and using leaves with petioles. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
