| 菜薹组织培养和植株再生研究 |
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| 引用本文： | 何晓明,潘瑞炽.菜薹组织培养和植株再生研究[J].上海农业学报,2001,17(2):37-40. |
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| 作者姓名： | 何晓明 潘瑞炽 |
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| 作者单位： | 1. 广东省农业科学院蔬菜研究所,
2. 华南师范大学生物系, |
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| 基金项目： | 广东省自然科学基金博士启动基金(974135)及广东省重点科技攻关项目的部分内容。 |
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| 摘 要： | 对6个菜薹品种的4种不同外植体进行了组织培养和植株再生研究，结果表明，子叶与茎尖中以分化不定芽，而下胚轴和幼根只能形成愈伤组织及不定根；在MS BA2mg/L NAA1mg/L ABA0.5mg/L AgNO34mg/L培养基上，子叶的不定芽分化频率最高（23．3％），而在MS＋BA1mg/L KT1mg/L NAA0.2mg/L培养基上，茎尖繁殖系统为1．66，快速繁殖效果较为理想，结果还表明，菜薹不同品种间的组织培养能力存在明显差异。
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| 关 键 词： | 菜薹 组织培养 植株再生 |
| 文章编号： | 1000-3924(2001)02-37-04 |
| 修稿时间： | 2000年8月14日 |
IN VITRO CULTURE AND PLANT REGENERATION OF
Brassica campestris L.ssp.chinensis var.utilis Tsen et Lee |
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| He Xiaoming,Pan Ruichi.IN VITRO CULTURE AND PLANT REGENERATION OF
Brassica campestris L.ssp.chinensis var.utilis Tsen et Lee[J].Acta Agriculturae Shanghai,2001,17(2):37-40. |
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| Authors: | He Xiaoming Pan Ruichi |
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| Abstract: | In vitro culture and plant regeneration were carried out in 6 varieties of Brassica campestris L.ssp.chinensis var.utilis Tsen et Lee. The results showed that adventitious buds were induced from shoot tips and cotyledons, while there were only calluses and roots formed in explants of hypocotyls and roots. Highest induced frequency (23.3%)of adventitious buds from cotyledons was obtained in medium MS+BA2mg/L +NAA1mg/L+ABA0.5 mg/L+AgNO3 4mg/L. Medium MS+BA1mg/L+KT1mg/L+NAA0.2mg/L was most suitable for rapid multiplication, in which the propagation coefficient of shoots was highest(1.66). The results also showed that there were differences in ability of differentiating adventitious buds among six test varieties. |
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