Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig
Oocytes Before Germinal Vesicle Breakdown |
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| Authors: | Tadatoshi Nobata Hirohisa Kyougoku Takashi Miyano |
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| Institution: | 1)Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan |
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| Abstract: | During oocyte growth, the morphology of the nucleolus changes into a compact and
homogenous structure. The compact nucleoli in full-grown oocytes are not stained by
aceto-orcein staining or immunofluorescence staining. In this study, we developed a
hematoxylin staining method for pig oocytes in whole-mount preparations to visualize
the nucleoli. Nucleoli of growing and full-grown oocytes were stained blue with
hematoxylin. Using this staining method, the changes in the oocyte nucleolus during
maturation were examined. The nucleolar diameter gradually decreased in maturing
oocytes (10.7 ± 0.1 μm to 9.0 ± 0.7 μm, P<0.05) before germinal vesicle breakdown
(GVBD). The results suggest that the nucleolar volume of oocytes decreases before
GVBD. |
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| Keywords: | Hematoxylin Maturation Nucleolus Oocyte Pig |
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