| 西藏核桃优良单株的FISH-AFLP分析 |
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| 引用本文： | 马庆国,王滑,潘刚,裴东.西藏核桃优良单株的FISH-AFLP分析[J].北京林业大学学报,2011,33(6):86-89. |
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| 作者姓名： | 马庆国 王滑 潘刚 裴东 |
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| 作者单位： | 1 中国林业科学研究院林业研究所，林木遗传育种国家重点实验室2 华中农业大学园艺林学学院3 西藏农牧学院西藏高原生态研究所 |
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| 基金项目： | 林业公益性行业科研专项(201004048) |
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| 摘 要： | 为了深入了解西藏核桃种质资源的遗传背景和遗传多样性,采用FISH-AFLP技术,筛选9对EcoRⅠ＋3和MseⅠ＋3引物组合,对我国西藏核桃主产区加查和郎县的11个核桃（JuglansregiaL.）优良单株进行基因组DNA水平检测。结果表明：在获得的1103条可统计条带中,1076条呈多态性,多态性达97.67%;9...
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| 关 键 词： | 核桃 遗传多样性 亲缘关系 AFLP 指纹图谱 |
| 收稿时间： | 1900-01-01 |
FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China. |
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| MA Qing-guo, WANG Hua , PAN Gang, PEI Dong.FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J].Journal of Beijing Forestry University,2011,33(6):86-89. |
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| Authors: | MA Qing-guo WANG Hua PAN Gang PEI Dong |
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| Institution: | 1 Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, P. R. China; 2 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, P. R. China; 3 Research Institute of Plateau Ecology, Tibet Agriculture and Animal Husbandry College, Linzhi, 860000, P. R. China. |
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| Abstract: | In order to deeply understand the genetic background and the genetic diversity of Tibet walnut germplasm resources, FISH AFLP was conducted using 9 EcoRⅠ+3/MseⅠ+3 primer combinations to analyse 11 walnut (Juglans regia L.) advanced trees from Tibet at genomic DNA level. Totally 1 103 AFLP fragments were obtained, in which 1 076 were polymorphic and the average percentage of polymorphic bands (ppb) was 97.67%. The value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 1.48±0.04; Neis gene diversity (H) ranged from 0.00 to 0.49 with an average of 0.29±0.02 and Shannons information index (I) ranged from 0.00 to 0.69 with an average of 0.44±0.02The genetic diversity and sibship among walnut advanced trees in Tibet were investigated and their fingerprintings were established in the research. |
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| Keywords: | Juglans regia L genetic diversity sibship AFLP fingerprinting |
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