| 大花红景天悬浮颗粒、悬浮细胞体系的研究 |
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| 引用本文： | 王彩云,丁友芳,翁慧,史玲玲,闫林林,刘玉军.大花红景天悬浮颗粒、悬浮细胞体系的研究[J].北京林业大学学报,2011,33(6):186-190. |
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| 作者姓名： | 王彩云 丁友芳 翁慧 史玲玲 闫林林 刘玉军 |
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| 作者单位： | 北京林业大学生物科学与技术学院，林木育种国家工程实验室 |
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| 基金项目： | “十一五”国家科技支撑计划重大专项(2009ZX09308-002) |
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| 摘 要： | 为了探讨大花红景天悬浮体系中红景天苷含量与其颗粒大小、生长状态的关系及可能机制，以大花红景天叶片为外植体，获得愈伤组织，建立悬浮颗粒体系，并分别通过60、80、100和120目细胞筛筛选进而建立悬浮细胞体系。测定了相同培养条件下悬浮颗粒(平均直径约2.5 cm)、悬浮细胞(过80目细胞筛所得)及二者相应培养液中红景天苷的含量。结果表明:经80目细胞筛过滤所得滤液培养一段时间后，可获得大量增殖速度较快、大小均一的悬浮细胞，且这些悬浮细胞中红景天苷含量达0.606 mg/g，约为悬浮颗粒中红景天苷含量0.27 mg/g的2.2倍。而悬浮颗粒和悬浮细胞相应培养液中则均未检测到红景天苷。悬浮体系中细胞与氧气、光照、营养成分的接触程度对培养物中红景天苷的含量有着非常重要的影响。
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| 关 键 词： | 大花红景天 悬浮颗粒 悬浮细胞 高效液相色谱 红景天苷 |
| 收稿时间： | 1900-01-01 |
Callus particles and cell suspension cultures of Rhodiola crenulata |
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| WANG Cai-yun,DING You-fang,WENG Hui,SHI Ling-ling,YAN Lin-lin,LIU Yu-jun..Callus particles and cell suspension cultures of Rhodiola crenulata[J].Journal of Beijing Forestry University,2011,33(6):186-190. |
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| Authors: | WANG Cai-yun DING You-fang WENG Hui SHI Ling-ling YAN Lin-lin LIU Yu-jun |
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| Institution: | College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, 100083, P.R.China. |
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| Abstract: | To investigate the relations between salidroside content and particle size as well as growth conditions, also the possible mechanism of Rhodiola crenulata, callus obtained from leaves of R. crenulata was used for raising particle suspension cultures, which were then filtered by cell sieves with 60, 80, 100 or 120 mesh respectively, so that cell suspension cultures were established. The salidroside content in particle suspension cultures (the average diameter was about 2.5 cm) and cell suspension cultures (filtered by 80 mesh cell sieve) were detected, both of the particle and cell culture solutions in the same culture conditions were also detected. After being filtered by 80 mesh cell sieve and then cultured for a period of time, the culture filtrate had been shown to produce vast rapid proliferated cell suspension cultures with uniform size. Cell suspension cultures had 0.606 mg/g of salidroside, which was about 2.2 times of that of particle suspension cultures (0.27 mg/g). However, neither of the solution of particle suspension cultures nor cell suspension cultures was found to have salidroside. It is concluded that salidroside content in suspension cultures has certain relations with particle size and growth condition of different cell aggregation. Since small cells contact fully with oxygen and nutrient of medium, they can produce high content of salidroside. |
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| Keywords: | Rhodiola crenulata particle suspention cultures cell suspension cultures high performance liquid chromatography(HPLC) salidroside |
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