A comparison of the potency of several Brucella allergens used to detect brucellosis in cattle |
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| Authors: | Z Bercovich T Dekker A Eger J Haagsma |
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| Institution: | (1) Department of Bacteriology, Institute for Animal Science and Health (ID-DLO), Edelhertweg 15, PO Box 65, 8200 AB Lelystad, The Netherlands |
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| Abstract: | The potency of Brucella allergens prepared from a smooth Brucella abortus strain S-99, mucoid strain Leewarden, rough strain 45/20, and rough Brucella melitensis strain B-115 was assessed. The potency of these allergens was compared with that of a standard allergen prepared from smooth Brucella abortus S-99 that efficiently detected bovine brucellosis in other studies. Eight cattle experimentally inoculated with Brucella abortus 544 were tested with the allergens 4 and 10 weeks after infection, and again 8 months after infection. All the allergens effectively detected infection but there was a clear distinction in the mean skin reactions 48 and 72 h after injection of the allergens. The skin reactions provoked by the allergens prepared from smooth or mucoid strains of Brucella were most pronounced 48 h after injection. Skin reactions provoked by allergens prepared from rough strains of Brucella were strongest 72 h after injection. Allergens prepared from smooth or mucoid Brucella strains were more potent in detecting brucellosis than those prepared from rough strains of Brucella.Abbreviations Bruc/OCB
Brucellergen OCB
- cfu
colony-forming units
- CFT
complement fixation test
- ID-DLO
Institute voor Dierhouderij en Diergezondheid-Dienst Landbouwkundig Onderzoek
- ICFTU
international complement fixation units
- IU
international units
- LPS
lipopolysaccharide
- SAT
serum agglutination test
- SDTH
skin delayed-type hypersensitivity |
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| Keywords: | allergen Brucella cattle delayed-type hypersensitivity diagnosis skin strain |
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