| 高效液相色谱法检测克氏原螯虾组织中氟苯尼考及氟苯尼考胺 |
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| 引用本文： | 岳刚毅,吴志新,于艳梅,杨倩,曲艺,谢玲玲,陈孝煊.高效液相色谱法检测克氏原螯虾组织中氟苯尼考及氟苯尼考胺[J].淡水渔业,2010,40(2). |
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| 作者姓名： | 岳刚毅 吴志新 于艳梅 杨倩 曲艺 谢玲玲 陈孝煊 |
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| 作者单位： | 华中农业大学水产学院,武汉,430070 |
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| 基金项目： | 国家科技支撑计划，湖北省科技攻关计划 |
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| 摘 要： | 建立了克氏原螯虾(Procambarus clarkii)血淋巴、肌肉、肝胰脏中氟苯尼考及氟苯尼考胺的高效液相色谱检测方法。采用Agilent Eclipse XDB-C18(250mm×4.6 mmi.d,5μm)反相色谱柱,以A液(乙腈):B液(0.04 mol/L NaH2PO4、0.004 mol/L十二烷基硫酸钠和0.10%三乙胺)(体积比为30∶70)为流动相,225 nm紫外检测波长完成分析。结果显示:本方法在0.025~20 mg/L范围内浓度与峰面积呈良好的线性关系;组织的加标回收率为78.8%~94.2%,相对标准偏差小于8.16%,氟苯尼考在血淋巴、肌肉、肝胰脏的检出限分别为0.01 mg/L、0.02 mg/kg、0.03 mg/kg,氟苯尼考胺则为0.015 mg/L、0.03 mg/kg、0.05 mg/kg。
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| 关 键 词： | 氟苯尼考 氟苯尼考胺 HPLC 克氏原螯虾(Procambarus clarkii) |
Simultaneous determination of florfenicol and florfenicol amine in tissues of Procambarus clarkii by high performance liquid chromatography |
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| YUE Gang-yi,WU Zhi-xin,YU Yan-mei,YANG Qian,QU Yi,XIE Ling-ling,CHEN Xiao-xuan.Simultaneous determination of florfenicol and florfenicol amine in tissues of Procambarus clarkii by high performance liquid chromatography[J].Freshwater Fisheries,2010,40(2). |
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| Authors: | YUE Gang-yi WU Zhi-xin YU Yan-mei YANG Qian QU Yi XIE Ling-ling CHEN Xiao-xuan |
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| Abstract: | A HPLC method was developed for simultaneous determination of florfenicol(FF)and its metabolite florfenicol amine(FFA)in the hemolymph,muscle and hepatopancreas of crayfish.The analysis column was reversed-phase column of Agilent Eclipse XDB-C18(250 mm×4.6 mm i.d,5 μm),the mobile phase was solution A(acetonitrile)and solution B(0.04 mol/L sodium dihydrogen phosphate,0.004 mol/L SDS and 0.10% triethylamine)(30∶70,V∶V),UV detector was set at 225 nm.The results showed that:the standard curves were linear in the investigated ranges of 0.025~20.0 mg/L,and the recovery values of florfenicol and florfenicol amine were from 78.8% to 94.2%,relative standard deviations were less than 8.16%.The limit of detection for florfenicol and florfenicol amine were 0.01 mg/L and 0.015 mg/L in hemolymph,0.02 mg/kg and 0.03 mg/kg in muscle,0.03 mg/kg and 0.05 mg/kg in hepatopancrea respectively. |
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| Keywords: | HPLC |
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