| 利用花序轴组培快繁青花菜萝卜胞质雄性不育系的研究* |
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| 引用本文： | 吴丽艳,柏柯帆,李石开,钟利,林良斌,和江明.利用花序轴组培快繁青花菜萝卜胞质雄性不育系的研究*[J].云南农业大学学报,2009,24(5):712-717. |
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| 作者姓名： | 吴丽艳 柏柯帆 李石开 钟利 林良斌 和江明 |
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| 作者单位： | [1]云南省农业科学院园艺研究所,云南昆明650205 [2]云南农业大学农学与生物技术学院,云南昆明650201 |
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| 基金项目： | 云南省科技攻关计划项目，云南省创新技术人才培养计划 |
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| 摘 要： | 以萝卜胞质不育型青花菜的幼嫩花序轴为外植体进行组织培养，可成功诱导出苗。将幼嫩花序轴接种到不同激素配比的诱导培养基上，均有愈伤组织产生，其中在MS + 6-BA 1.0mg/L + NAA 0.2mg/L培养基培养效果最好，诱导率高达100%；用MS + NAA 0.01mg/L +KT 0.2mg/L + 6-BA 0.5mg/L分化不定芽时，不定芽分化效果最好，芽分化率可达6.16；用1/2 MS + NAA 0.2mg/L进行生根培养，生根率高达86.7%，平均根数13.5条，在一定的温度和湿度条件下培养，移栽成活率可达85%以上。
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| 关 键 词： | 青花菜 花序轴 组培快繁 萝卜胞质雄性不育系 |
| 收稿时间： | 2008-11-03 |
Studies on Tissue Culture of Rachides and Consequent Rapid Multiplication of OguCMS Lines in Brassica oleracea L. var. varitalica Planch |
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| WU Li-yan,BAI Ke-fan,LI Shi-kai,ZHONG Li,LIN Liang-bin,HE Jiang-ming.Studies on Tissue Culture of Rachides and Consequent Rapid Multiplication of OguCMS Lines in Brassica oleracea L. var. varitalica Planch[J].Journal of Yunnan Agricultural University,2009,24(5):712-717. |
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| Authors: | WU Li-yan BAI Ke-fan LI Shi-kai ZHONG Li LIN Liang-bin HE Jiang-ming |
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| Institution: | 1. Horticultural Institute, Yunnan Academy of Agricultural Science, Kunming 650205, China; 2. College of Agricultural Sciences and Biotechnology, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Tissue culture of OguCMS Brassica oleraceaL. var. varitalica Planch, which was done with its young rachides as explants, succeeded in seedling. After inoculating the young rachides against the induction medium compounded with different hormones, and it had the uppermost rachis inducing rate of 100% on the induction medium (MS + 6-BA 1.0mg/L + NAA 0.1mg/L). Seedlings showed favorable growth after being cultivated consequently on the enrichment medium (MS + 6-BA 1.0mg/L + NAA 0.01mg/L ) with a proliferation coefficient of 6.16. The rhizogenic rate was 86.7% with 13.5 pieces of roots in average on the rooting medium (1/2MS + NAA 0.2mg/L). Seedlings survival rate was up to 85% in certain temperature and humidity condition. |
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| Keywords: | broccoli rachis tissue culture and rapid multiplication OguCMS lines |
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