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| Ca~(2+)信号介导川芎嗪诱导小鼠骨髓间充质干细胞向神经细胞的定向分化 | |
| 引用本文： | 刘云云,赵兴绪,赵红斌,葛宝丰,刘兴炎,陈克明.Ca~(2+)信号介导川芎嗪诱导小鼠骨髓间充质干细胞向神经细胞的定向分化[J].甘肃农业大学学报,2010,45(2). |
| 作者姓名： | 刘云云 赵兴绪 赵红斌 葛宝丰 刘兴炎 陈克明 |
| 作者单位： | 1. 甘肃农业大学生命科学技术学院,甘肃,兰州,730070 2. 甘肃农业大学动物医学院,甘肃,兰州,730070 3. 兰州军区兰州总医院,甘肃,兰州,730050 |
| 基金项目： | 甘肃省自然科学基金(3ZS051-A25-078) |
| 摘 要： | 用含2.4 mg.mL-1川芎嗪提取液对小鼠骨髓间充质干细胞(BMSCs)进行诱导,探讨川芎嗪体外诱导BMSCs分化为神经元样细胞的作用.应用EGTA(细胞外Ca2+螯合剂)、Nifedipine(L-型Ca2+通道阻断剂)和LY294002(PI3K阻断剂)等Ca2+阻断剂分别作用细胞,RT-PCR和Western blot技术研究Ca2+信号在川芎嗪诱导BMSCs分化为神经细胞过程中的作用.结果表明:川芎嗪作用不同时间的BMSCs均可见Nestin、β-Tubulin Ⅲ、NSE和Nurrl的表达;川芎嗪诱导后的细胞浆内Nestin和NSE蛋白表达呈阳性.EGTA、Nifedipine及LY294002分别阻断细胞外Ca2+、L-型Ca2+通道及PI3K后,NSE和Nurr1基因及NSE蛋白表达较川芎嗪诱导组显著上调.以上结果说明川芎嗪能使BMSCs定向分化为神经元样细胞,细胞内、外Ca2+的减少可促进川芎嗪诱导BMSCs向神经细胞的分化,Ca2+信号在川芎嗪诱导BMSCs向神经细胞定向分化过程中起负调控作用. |
| 关 键 词： | 川芎嗪 骨髓间充质干细胞(BMSCs) 神经元样细胞 Ca2+信号 |
Tetramethylpyrazine induces the differentiation of mouse bone marrow-derived mesenchymal stem cells into nerve cells mediated by Ca2+ signaling |
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| LIU Yun-yun,ZHAO Xing-xu,ZHAO Hong-bin,GE Bao-feng,LIU Xing-yan,CHEN Ke-ming.Tetramethylpyrazine induces the differentiation of mouse bone marrow-derived mesenchymal stem cells into nerve cells mediated by Ca2+ signaling[J].Journal of Gansu Agricultural University,2010,45(2). | |
| Authors: | LIU Yun-yun ZHAO Xing-xu ZHAO Hong-bin GE Bao-feng LIU Xing-yan CHEN Ke-ming |
| Institution: | 1.College of Life Science and Technology;Gansu Agricultural University;Lanzhou 730070;China;2.College of Veterinary Medicine;3.Lanzhou Generation Hospital of Lanzhou Military Area Command;Lanzhou 730050;China |
| Abstract: | Mouse bone marrow-derived mesenchymal stem cells(BMSCs) were induced by the induction medium containing 2.4 mg·mL-1 tetramethylpyrazine to investigate the ability of BMSCs to differentiate into neuron-like cells in vitro,and the blocking agents,such as EGTA(Ca2+ chelator),Nifedipine(L-type Ca2+ channel blocker)and LY294002(PI3K inhibitor),were applied to block the cellular Ca2+ signal pathway to further study the effect of Ca2+ signal on the neural differentiation of BMSCs induced by tetramethylpyrazine with RT-PCR and Western blot methods.RT-PCR analysis revealed that an expression of several neural genes including Nestin,β-Tubulin III,NSE and Nurrl were found in the induced BMSCs.The expression of several neural specific proteins,such as Nestin and neuron-specific enolase(NSE) were detected by Western blot method.NSE and Nurr1 were conspicuously up-regulated after the Ecto Ca2+,L-type Ca2+ channel and PI3K blocked respectively by EGTA,Nifedipine and LY294002.These results suggested that tetramethylpyrazine can induce the BMSCs differentiate into nerve cells,the reduction of ectocellular or intracellar Ca2+ can contribute to neural differentiation,so the Ca2+ signaling pathway play an important negative regulation role in the neural differentiation of BMSCs induced by tetramethylpyrazine. |
| Keywords: | tetramethylpyrazine bone marrow-derived mesenchymal stem cell neuron-like cells Ca2+ signaling |
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