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| 基于草甘膦筛选的菘蓝转化体系的建立 | |
| 引用本文： | 唐俊,张富生,廖志华.基于草甘膦筛选的菘蓝转化体系的建立[J].江西农业学报,2011,23(3). |
| 作者姓名： | 唐俊 张富生 廖志华 |
| 作者单位： | 1. 江西省南昌市食品质量卫生安全监督检验中心,江西,南昌,330012;西南大学,生命科学学院、三峡库区生态环境教育部重点试验室,重庆400715 2. 江西省农产品质量安全检测中心,江西,南昌,330046 3. 西南大学,生命科学学院、三峡库区生态环境教育部重点试验室,重庆400715 |
| 摘 要： | 介绍了以菘蓝草甘膦筛选的遗传转化体系,以携带植物表达载体pASM12的根癌农杆菌LBA4404对四倍体菘蓝进行叶盘法转化,在选择培养基中加入8 mg/L的草甘膦进行筛选,获得草甘膦抗性的再生植株,对再生植株进行PCR检测和草甘膦抗性试验,结果表明:PCR检测表明子叶的转化频率达30%,下胚轴的转化频率达40%;对PCR阳性植株的草甘膦抗性试验表明,多数植株在不同程度上表现出对草甘膦的抗性。 |
| 关 键 词： | 菘蓝 根癌农杆菌 遗传转化 草甘膦 |
Establishment of Transformation System of Isatis indigotica by Using Glyphosate as Selectable Marker |
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| TANG Jun,ZHANG Fu-sheng,LIAO Zhi-hua.Establishment of Transformation System of Isatis indigotica by Using Glyphosate as Selectable Marker[J].Acta Agriculturae Jiangxi,2011,23(3). | |
| Authors: | TANG Jun ZHANG Fu-sheng LIAO Zhi-hua |
| Institution: | 1.Nanchang Testing Center of Agricultural Products Quality and Safety in Jiangxi Province; Nanchang 330012; China; 2.Key Laboratory of Eco-environment in Three Gorges Reservoir Region; Ministry of Education; School of Life Sciences; Southwest University; Chongqing 400715; 3.Jiangxi Provincial Testing Center of Agricultural Products Quality and Safety; Nanchang 330046; China); |
| Abstract: | The transformation system of Isatis indigotica by using Glyphosate as the selectable marker was established in this study.Tetraploid Isatis indigotica was transformed via Agrobacterium tumefaciens strain LBA4404 carrying the plasmid pASM12.The transgenic explants were directly selected on the media containing 8 mg/L Glyphosate,and the transgenic Glyphosate-resistant plantlets were confirmed by genomic PCR techniques and Glyphosate resistance test.PCR analysis showed the transformation frequency of the cotyl... |
| Keywords: | Isatis indigotica Agrobacterium tumefaciens Genetic transformation Glyphosate |
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