| 南疆扁桃品种自交不亲和S-Rnase基因型鉴定 |
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| 引用本文： | 姜俊卿,冯建荣,曹晓艳,李文惠,王雪莲,陈玲.南疆扁桃品种自交不亲和S-Rnase基因型鉴定[J].新疆农业科学,2011,48(7):1177-1182. |
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| 作者姓名： | 姜俊卿 冯建荣 曹晓艳 李文惠 王雪莲 陈玲 |
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| 作者单位： | 石河子大学农学院,新疆石河子,832003;新疆农业科学院轮台国家果树资源圃,新疆轮台,841600 |
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| 基金项目： | 新疆生产建设兵团科技攻关计划(2D2007GG03) |
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| 摘 要： | 目的]鉴定南疆扁桃品种自交不亲和S-RNase基因型,可为科学合理地配置授粉品种提供依据,有效提高南疆扁桃的产量.方法]以南疆10个扁桃品种的叶片为试材,利用蔷薇科通用引物PaConsⅡ-F+PaConsⅡ-R、EM - PC2consFD+ EM - PC3consRD对叶片基因组DNA进行S-RNase特异性扩增,将克隆测序结果在GenBank上进行同源性比对.结果]显示10个扁桃品种均获得2条带,共20条带,包含16种不同的S基因核苷酸序列,其中4个为GenBank上登录的已知S -RNase基因序列,分别为S1(1 370 bp)、S12(2 479bp)、S14(740bp)、S52(1 626bp),12个为新的S-RNase基因序列,暂时分别命名为SA(780bp)、SB(530bp)、SC(1261 bp)、SD(432 bp)、SE(1266bp)、5F(270 bp)、SG(1263 bp)、SH(272 bp)、SI(690 bp)、SJ(1 523bp)、SK(755bp)、SL(1 486bp).结论]鉴定出10个品种的S- RNase基因型.
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| 关 键 词： | 扁桃 自交不亲和 S基因型 |
| 收稿时间： | 2011-07-25 |
Identification of Self- Incompatibility S - RNase Genotypes for Almond Cultivars in Xinjiang |
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| JIANG Jun-qing,FENG Jian-rong,CAO Xiao-yan,LI Wen-hui,WANG Xue-lian,CHEN Ling.Identification of Self- Incompatibility S - RNase Genotypes for Almond Cultivars in Xinjiang[J].Xinjiang Agricultural Sciences,2011,48(7):1177-1182. |
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| Authors: | JIANG Jun-qing FENG Jian-rong CAO Xiao-yan LI Wen-hui WANG Xue-lian CHEN Ling |
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| Institution: | JIANG Jun-qing1,FENG Jian-rong1,CAO Xiao-yan1,LI Wen-hui2,WANG Xue-lian1,CHEN Ling1(1.College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China,2.Luntai National Fruit Germplasm Resource Garden,Xinjiang Academy of Agricultural Sciences,Luntai Xinjiang 841600,China) |
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| Abstract: | 【Objective】The purpose of this program was to identify S-RNase genotypes associated with self-incompatibility of almond cultivated in Xinjiang.【Method】 2 pairs of consensus primer PaConsⅡ F + PaConsⅡ-R and EM-PC2consFD + EM-PC3consRD which had been published for S-RNase in prunus were used to amplify genome DNAs of the leaves from 10 almond cultivars.The specific fragments were cloned and sequenced,and then the nucleotide acid sequences were blasted on GenBank.【Result】The results showed: 10 Almond varieties were amplified and obtained bands with a total of 20,Searches in GenBank for homology with our sequences publicly available DNA databases revealed that 10 almond varieties contained 16 different S-RNase genes,and 4 S-RNase gene fragments had been deposited in the GenBank as S1(1 370 bp),S12(2 479 bp), S14(740 bp),S52(1 626 bp) and 12 SRNase gene fragments were novel S-RNase genes,temporarily named as SA(780 bp),SB(530 bp),SC(1 261 bp),SD(432 bp),SE(1 266 bp),SF(270 bp),SG(1 263 bp), SH(272 bp),SI(690 bp),SJ(1 523 bp),SK(755 bp),SL(1 486 bp).【Conclusion】Ten S-Rnase were identified in the research. |
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| Keywords: | almond self-incompatibility S-genotypes |
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