| 不同培养体系对绵羊体外受精胚胎发育及细胞凋亡的影响 |
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| 引用本文： | 万鹏程,石文艳,代蓉,周平,刘守仁,石国庆.不同培养体系对绵羊体外受精胚胎发育及细胞凋亡的影响[J].新疆农业科学,2011,48(7):1300-1306. |
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| 作者姓名： | 万鹏程 石文艳 代蓉 周平 刘守仁 石国庆 |
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| 作者单位： | 新疆农垦科学院畜牧兽医研究所,新疆石河子832000;新疆兵团绵羊繁育生物技术重点实验室,新疆石河子832000;石河子大学医学院生理教研室,新疆石河子,832000 |
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| 基金项目： | 转基因重大专项(2009ZX08008-001B); 新疆生产建设兵团博士基金(2010JC10); 兵团院士基金(2011BB024); 新疆农垦科学院青年基金(YQJ2009-01); 国家绒毛用羊产业技术体系(nycytx-40-06); 新疆生产建设兵团绵羊繁育生物技术重点实验室开放课题(05KLS04) |
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| 摘 要： | 目的]通过SOFaaBSA和CR1 aaBSA - FBS两种培养液对体外胚胎发育率及胚胎凋亡发生的影响,筛选适合绵羊体外受精胚胎发育的培养体系,为相关研究提供一定的理论依据和研究基础.方法]采用激光共聚焦显微镜和细胞原位凋亡检测技术(TUNEL),分析这两种胚胎体外培养系统的绵羊体外受精胚胎发育的影响及各发育阶段的细胞凋亡状况及对胚胎发育的影响.结果]SOFaaBSA和CR1aaBSA- FBS组的卵裂率、囊胚率和囊胚孵化率均高与其它实验组,但这两组之间差异不显著(P＞0.05).同时,在两种培养液培养的2细胞、3-8细胞期的绵羊体外受精正常形态的胚胎中均未发现凋亡信号,凋亡信号在两种培养液中都是首次出现在9- 16细胞胚胎细胞中,并且随着胚胎发育至囊胚期,凋亡细胞效随着胚龄增长越来越多.在SOFaaBSA培养液培养的桑椹胚和囊胚细胞胚胎凋亡率显著低于CR1aaBSA - FBS培养液培养的桑椹胚和囊胚(P＜0.05),同时,在CR1aaBSA- FBS培养液中桑椹胚和囊胚细胞平均具有凋亡细胞的数量显著高于SOFaaBSA培养液(P＜0.05).结论]CR1aaBSA- FBS培养系统显著的增加了绵羊晚期体外胚胎的凋亡.CR1 aaBSA - FBS能够支持绵羊体外受精胚胎的发育,但SOFaaBSA培养液更适合绵羊体外胚胎的发育.
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| 关 键 词： | 绵羊 体外受精 凋亡 TUNEL |
| 收稿时间： | 2011-07-25 |
Effects of SOF and CR1 Cultural Systems on Developmental Competence and Cell Apoptosis of Ovine Embryo Derived in Vitro |
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| WAN Peng-cheng,SHI Wen-yan,DAI Rong,ZHOU Ping,LIU Shou-ren,SHI Guo-qing.Effects of SOF and CR1 Cultural Systems on Developmental Competence and Cell Apoptosis of Ovine Embryo Derived in Vitro[J].Xinjiang Agricultural Sciences,2011,48(7):1300-1306. |
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| Authors: | WAN Peng-cheng SHI Wen-yan DAI Rong ZHOU Ping LIU Shou-ren SHI Guo-qing |
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| Institution: | WAN Peng-cheng1,2,SHI Wen-yan3,DAI Rong1,ZHOU Ping1,LIU Shou-ren1,SHI Guo-qing1,2 (1 Animal Husbandry and Veterinary Institute,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi Xinjiang 832000,China,2 Key Lab of Sheep Breeding and Reproduction,Xinjiang Agroreclamation Academy of Sciences,3 Medical College,Shihezi University,China) |
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| Abstract: | 【Objective and Method】The study was to investigate effects of synthetic oviduct fluid(SOF) and Charles Rosenkrans medium(CR1) culture systems on developmental competence and cell apoptosis of ovine in vitro fertilization (IVF) embryos.Ovine presumptive IVF zygotes were cultured in the following two media:(1) SOF supplemented with amino acids(SOFaa) and 8 mg/mL bovine serum albumin(BSA) for nine days(SOFaaBSA);(2) CR1aa supplemented with 10% FBS for nine days(CR1aaFBS).【Result】The results show that the rates of blastocyst and hatched blastocyst in two groups were not different(P > 0.05).Both numbers of total cell and trophectoderm(TE) in expanded or hatched blastocyst from SOFaaBSA were significantly higher than CR1aaBSA-FBS(P < 0.05).The apoptotic signals were firstly observed at 8-cell stage in two groups and became stronger and stronger with the development of embryos.Rates of embryos with apoptotic signals in group 2 at morula or blastocyst were greater than that in group 1(P < 0.05).The apoptotic nuclei numbers of morula or blastocyst in group 2 were also significantly higher than that in group 1(P < 0.05).【Conclusion】It is concluded that CR1aaBSA-FBS can support development of ovine IVF embryos in vitro,but SOFaaBSA is more suitable. |
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| Keywords: | Ovine in vitro fertilization apoptosis TUNEL |
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