| 黄瓜前中期染色体C - 分带及其制备方法研究 |
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| 引用本文： | 钱春桃,陈劲枫,罗向东,曹清河,雷春.黄瓜前中期染色体C - 分带及其制备方法研究[J].园艺学报,2004,34(6):814-816. |
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| 作者姓名： | 钱春桃 陈劲枫 罗向东 曹清河 雷春 |
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| 作者单位： | (作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院, 南京210095) |
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| 基金项目： | 国家高技术研究发展计划专项(2002AA241251,2002AA207012)，国家自然科学基金资助项目(30170644,30470120)，教育部跨世纪优秀人才培养计划项目，南京农业大学青年科技创新基金项目(KJ04009) |
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| 摘 要： | 以活体种子根为材料, 采用放线菌酮预处理, 直接获得了清晰的黄瓜前中期染色体C - 分带。黄瓜前中期染色体的长度介于3～8μm , 单套染色体组具有21 条稳定的C - 带, 包括12 条末端带、7 条着丝点带、1 条中间带和1 条随体带。还探讨了影响前中期C - 分带制备的关键因素, 提出了黄瓜前中期染色体C - 分带的实用制备方法。
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| 关 键 词： | 黄瓜 染色体 C - 分带 前中期 |
| 文章编号： | 0513-353X(2004)06-0814-03 |
| 收稿时间： | 2004-2-2 |
| 修稿时间： | 2004-4-5 |
C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation |
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| Qian Chuntao,Chen Jinfeng ,Luo Xiangdong,Cao Qinghe,and Lei Chun.C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation[J].Acta Horticulturae Sinica,2004,34(6):814-816. |
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| Authors: | Qian Chuntao Chen Jinfeng Luo Xiangdong Cao Qinghe and Lei Chun |
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| Institution: | ( State Key Laboratory of Crop Genetics and Germplasm Enhancement , College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China) |
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| Abstract: | Clear C-bands at prometaphase were obtained in cucumber ( Cucumis sativus L. , 2n = 2x = 14) when cycloheximide was applied to the germinating roots in vivo as pretreatment . Chromosome length at prometaphase varied from 3 to 8μm. Haploid complement had 21 distinct and stable C-bands , including 12 telomeric - , 7 centromeric - , 1 intercary - and 1 satellite-bands. Some factors affecting the band formation were investigated and a practical procedure of C-banding of prometaphase chromosome was proposed. |
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| Keywords: | Cucumber Chromosome C-banding Prometaphase |
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