| 16种商品海参16S rRNA的PCR-RFLP鉴定方法 |
| |
| 引用本文： | 文菁,胡超群,张吕平,范嗣刚.16种商品海参16S rRNA的PCR-RFLP鉴定方法[J].中国水产科学,2011,18(2):451-457. |
| |
| 作者姓名： | 文菁 胡超群 张吕平 范嗣刚 |
| |
| 作者单位： | 1. 湛江师范学院,生命科学与技术学院,广东,湛江,524048;中国科学院,南海海洋研究所,中国科学院海洋生物资源可持续利用重点实验室,广东省应用海洋生物学重点实验室,广东,广州,510301
2. 中国科学院,南海海洋研究所,中国科学院海洋生物资源可持续利用重点实验室,广东省应用海洋生物学重点实验室,广东,广州,510301 |
| |
| 基金项目： | 国家科技支撑计划项目，广东省海洋渔业科技项目，广东省科技计划项目，广东省省院合作计划项目，广西自治区重点科技项目 |
| |
| 摘 要： | 基于570 by左右的线粒体16S rRNA基因片段,利用3种核酸内切酶(Dde I,Hae III和Sty I)对16种海参PCR产物进行酶切,分别产生10种、5种和5种单倍型,通过单倍型的组合,即能有效区分16种海参的种类.运用此方法对19种商品海参(包括冻品和干品)进行鉴定,结果表明,9种产品属于错误贴标.本研究建立的PCR-RFLP方法方便、有效,可靠,可为海参产品评估、进出口种类鉴定提供实用高效的方法.本研究旨在为市场海参产品贴标情况的评估提供技术支撑.
|
| 关 键 词： | 海参 16S rRNA基因 鉴定方法 |
| 修稿时间： | 2011/3/10 0:00:00 |
PCR-RFLP identification of 16 commercial sea cucumber species on the basis of 16S rRNA gene |
| |
| WEN Jing,HU Chaoqun,ZHANG Lüping,FAN Sigang.PCR-RFLP identification of 16 commercial sea cucumber species on the basis of 16S rRNA gene[J].Journal of Fishery Sciences of China,2011,18(2):451-457. |
| |
| Authors: | WEN Jing HU Chaoqun ZHANG Lüping FAN Sigang |
| |
| Institution: | WEN Jing 1,2,HU Chaoqun 2,ZHANG Lüping 2,FAN Sigang 2 1.Department of Biology,Zhanjiang Normal University,Zhanjiang 524048,China,2.Key Laboratory of Marine Bio-resources Sustainable Utilization,Guangdong Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301 |
| |
| Abstract: | PCR-RFLP methodology was developed as a tool to assess the incidence of incorrect labeling of sea cucumbers in commercial food products. The technique allows the genetic identification of 16 species of sea cucumber based on PCR amplification of a common 570 bp fragment of the 16S rRNA gene and follows digestion with 3 restriction enzymes (types, respectively. The method was applied to authenticate the exact species of 19 commercial samples, including frozen and dried products. The results showed that 9 samples were incorrectly labeled (47%). Therefore, PCR-RFLP tool really provides convenient, useful and academic approach to identify commercial sea cucumber and makes the traceability to be possible. |
| |
| Keywords: | PCR-RFLP |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
