| 大豆品种成熟期基因型推测的研究 |
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| 引用本文： | 宁慧霞,李英慧,刘章雄,常汝镇,关荣霞,罗淑萍,邱丽娟.大豆品种成熟期基因型推测的研究[J].作物学报,2008,34(3):382-387. |
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| 作者姓名： | 宁慧霞 李英慧 刘章雄 常汝镇 关荣霞 罗淑萍 邱丽娟 |
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| 作者单位： | 1 中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程 /农业部作物品种资源与生物技术重点开放实验室，北京100081；2 新疆农业大学，新疆乌鲁木齐830052 |
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| 基金项目： | 国家自然科学基金
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国家高技术研究发展计划(863计划)
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国家科技支撑计划 |
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| 摘 要： | 选择不同来源中国大豆品种23份和国外引进的成熟期近等基因系35份进行SSR分析，目的是鉴定与成熟期基因型紧密连锁的标记，进而推测中国大豆品种的成熟期基因型。结果表明，（1）210对SSR标记中125对在成熟期近等基因型中具有多态性，推测与成熟期有关的标记有8个；（2）在Clark近等基因系中，筛选出成熟期基因E3/e3特异性标记Satt229，E4/e4的特异SSR标记Sct_010、Satt294、Satt247、Satt452和Satt156；在Clark和Harosoy近等基因系中，筛选出E7/e7的特异性SSR标记为Satt071、Satt178；（3）根据8个与成熟期相关的标记的分子数据，构建了国外大豆近等基因系的UPGMA聚类图，共聚为4类，背景来源相同或相似的材料被聚为一类，明显分为Clark近等基因系和Harosoy近等基因系。（4）与近等基因系成熟期基因(E7)分子标记比对，推测出25份中国大豆品种的成熟期基因。
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| 关 键 词： | 大豆 成熟期 近等基因系 SSR分子标记 |
| 收稿时间： | 2007-05-01 |
| 修稿时间： | 2007年5月1日 |
Deducing Maturity Genotype of the Chinese Soybean Varieties |
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| NING Hui-Xia,LI Ying-Hui,LIU Zhang-Xiong,CHANG Ru-Zhen,GUAN Rong-Xia,LUO Shu-Ping,QIU Li-Juan.Deducing Maturity Genotype of the Chinese Soybean Varieties[J].Acta Agronomica Sinica,2008,34(3):382-387. |
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| Authors: | NING Hui-Xia LI Ying-Hui LIU Zhang-Xiong CHANG Ru-Zhen GUAN Rong-Xia LUO Shu-Ping QIU Li-Juan |
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| Institution: | 1.The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) /Key Laboratory of Germplasm & Biotechnology (MOA) /Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;2.College of Agronomy, Xinjiang Agricultural University, Urumchi 830052, Xinjiang, China |
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| Abstract: | In order to find the tightly linked diagnostic SSR (simple sequence repeat) marker on soybean LG related to the maturity genotype and further deduce the maturity genotype of Chinese varieties, 215 pairs of SSR marker were selected to analyze 23 soybean germplasms in China and 35 introduced maturity Near-Isogenic Lines from U.S.A. Among them, 125 SSR markers were polymorphic and 8 SSR markers were relative to the maturity genotype. In detail, on the Clark background, Satt229 tightly linked to E3/e3 maturity locus, five markers including Sct_010, Satt294, Satt247, Satt452, and Satt156 tightly linked to E4/e4 maturity locus; both on the Clark and Harosoy background, Satt071and Satt178 tightly linked to E7/e7 maturity locus. Anyway, considered the NILs in the Harosoy background, only two markers (Satt071and Satt178) could be used to deduce the genotype of soybean germplasms in China. Then the maturity genotype of 25 varieties from China was deduced. UPGMA clustering tree was constructed based on the molecular data of 8 SSR markers correlated to maturity gene, and there were four groups were clustered. The result showed that the materials with the same genetic background were clustered together. The materials with the Clark background were clearly different from those with the Harosoy background. |
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| Keywords: | Soybean Maturity Near-Isogenic Lines SSR markers |
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