| HPLC法测定恩诺沙星可溶性粉含量 |
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| 引用本文： | 谢玉荣,吴少玲,李强,张滢.HPLC法测定恩诺沙星可溶性粉含量[J].家畜生态,2013(10):51-54. |
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| 作者姓名： | 谢玉荣 吴少玲 李强 张滢 |
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| 作者单位： | 海南省兽药饲料监察所,海南海口570203 |
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| 摘 要： | 采用HPLC法测定恩诺沙星可溶性粉的含量,色谱柱选用Waters Symmetry C18柱（4.6×150mm,5μm）,流动相为0.025 mol/L磷酸（用三乙胺调节pH至3.0）—乙腈（83∶17）,检测波长为278 nm,柱温25℃,流速1.0 mL/min.结果表明恩诺沙星在20～80 μg/mL的范围内,呈现良好的线性关系,平均回收率为99.3％.通过试验确定本法的检测限为15 μg/mL,定量限为50 μg/mL,且具有良好的专属性和耐用性.运用本法对来自四个不同厂家的恩诺沙星可溶性粉进行含量测定,并与紫外分光光度法测定结果比较,说明HPLC法能准确测定恩诺沙星可溶性粉的含量.
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| 关 键 词： | 恩诺沙星 高效液相色谱法 含量测定 |
Determination of Enrofloxacin Soluble Powder by HPLC |
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| XIE Yu-rong,WU Shao-ling,LI Qiang,ZHANG Ying.Determination of Enrofloxacin Soluble Powder by HPLC[J].Ecology of Domestic Animal,2013(10):51-54. |
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| Authors: | XIE Yu-rong WU Shao-ling LI Qiang ZHANG Ying |
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| Institution: | ( Hainan Provincial Institute of Veterinary Drug and Feed Control, Haikou , Hainan 570203, China) |
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| Abstract: | A HPLC method for the determination of enrofloxacin soluble powder has been used on Waters Symmetry C18 column（4.6 × 150 mm,5 μm）,with 0.025 mol/L phosphoric acid （adjust pH to 3.0 by triethylamine）-acetonitrile （83 ∶ 17） as mobile phase,UV detection at 278 nm,the column temperature was 25 ℃ and flow rate was 1.0 mL/min.The method showed good specificity,durability and linear relationship within the range of 20 ～ 80μg/mL.LOD and LOQ of enrofloxacin were determined as 15ng/mL and 50 ng/mL.The average recovery of enrofloxacin was 99.3 %.Determination of enrofloxacin soluble powder from four different manufacturers by HPLC and UV spectrophotometry indicated that the HPLC method can accurately control the quality of enrofloxacin soluble powder. |
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| Keywords: | enrofloxacin HPLC determination |
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