| 内蒙古地区西门塔尔牛亲子鉴定微卫星标记筛选 |
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| 引用本文： | 宋玲,贺建宁,孙东晓,李俊雅,张沅.内蒙古地区西门塔尔牛亲子鉴定微卫星标记筛选[J].中国畜牧杂志,2011,47(13). |
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| 作者姓名： | 宋玲 贺建宁 孙东晓 李俊雅 张沅 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193
2. 中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 国家高技术863计划(2008AA10Z146); “十一五”国家科技支撑计划(2006BAD01A10) |
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| 摘 要： | 研究旨在筛选适合于我国肉牛亲子鉴定的微卫星标记,以内蒙古乌拉盖地区368头西门塔尔牛为实验材料,利用荧光引物PCR和ABI3730测序仪对12个微卫星标记进行基因型检测,应用Cervus3.0软件统计12个微卫星标记的遗传多态性。结果表明:其中4个微卫星标记扩增效率较低,被剔除;其他8个微卫星的多态性丰富,平均等位基因数为11.38,平均期望杂合度为0.6686,平均多态信息含量为0.6354,适合于我国肉牛亲子鉴定。
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| 关 键 词： | 微卫星 亲子鉴定 西门塔尔牛 |
Identification of Microsatellite Markers for Simmental Paternity Testing |
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| SONG Ling,HE Jian-ning,SUN Dong-xiao,LI Jun-ya,ZHANG Yuan.Identification of Microsatellite Markers for Simmental Paternity Testing[J].Chinese Journal of Animal Science,2011,47(13). |
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| Authors: | SONG Ling HE Jian-ning SUN Dong-xiao LI Jun-ya ZHANG Yuan |
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| Institution: | SONG Ling1,HE Jian-ning2,SUN Dong-xiao1,LI Jun-ya2,ZHANG Yuan1(1.College of Animal Science and Technology,China Agricultural University,Beijing 100193,China,2.Institute of Animal Science,Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | To identify the microsatellite markers suitable to paternity testing of Chinese beef population,in this study,12 microsatellites were selected and genotyped with fluorescent dye-labelled PCR primers(5'-FAM or HEX) and ABI3730 DNA Sequencer in 368 Simmental of Mongolia ulgai.Cervus3.0 program was used to calculate the polymorphism of the 12 microsatellites.The results showed that the efficiency of PCR amplification was low in 4 microsatellites which were deleted.The other 8 microsatellites were highly polymo... |
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| Keywords: | microsatellite markers paternity testing Simmental cattle |
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