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| 不同生态环境下冬小麦籽粒大小相关性状的QTL分析 | |
| 引用本文： | 王瑞霞,张秀英,伍玲,王瑞,海林,游光霞,闫长生,肖世和.不同生态环境下冬小麦籽粒大小相关性状的QTL分析[J].中国农业科学,2009,42(2):398-407. |
| 作者姓名： | 王瑞霞 张秀英 伍玲 王瑞 海林 游光霞 闫长生 肖世和 |
| 作者单位： | 1. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京,100081;泰安市农业科学研究院,泰安,271000 2. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京,100081 3. 四川省农业科学院作物研究所,成都,610066 4. 安徽省农业科学院作物研究所,合肥,230036 |
| 基金项目： | 农业部农业结构调整重大技术研究专项项目 |
| 摘 要： | 【目的】鉴定影响籽粒大小相关性状的QTL，并估计QTL的表型效应;分析不同环境下QTL的稳定性。【方法】以冬小麦小粒地方品种和尚麦为母本，大粒育成品种豫8679为父本及其F7:8重组自交系的142个株系为试验材料，分析籽粒长度、宽度、厚度、体积及千粒重在北京（2006、2007）、合肥（2007）和成都（2007）4个不同环境下的性状表现，并利用已构建的含有170个SSR标记和2个EST标记的遗传图谱，对这5个性状进行QTL定位分析。【结果】4个环境下共检测到93个影响籽粒长度、宽度、厚度、体积及千粒重的QTL，这些QTL分布在除1D和6A之外的所有小麦染色体上。在检测到的QTL中，与籽粒长度、宽度、厚度、体积和千粒重相关的QTL分别为17、16、18、21和21个。另外，本研究还在1A、1B、2A、2D、3A、3B、5A、5B、5D、6A、6D、7B和7D染色体上共发现了18个QTL富集区。【结论】获得93个影响小麦籽粒大小相关性状的QTL，这些QTL可作为利用分子标记辅助育种途径进行小麦遗传改良的依据。 |
| 关 键 词： | 普通小麦 QTL 重组自交系 粒长 粒宽 粒厚 籽粒体积 千粒重 |
| 收稿时间： | 2008-1-22 |
QTL Analysis of Grain Size and Related Traits in Winter Wheat Under Different Ecological Environments |
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| WANG Rui-xia,ZHANG Xiu-ying,WU Ling,WANG Rui,HAI Lin,YOU Guang-xia,YAN Chang-sheng,XIAO Shi-he.QTL Analysis of Grain Size and Related Traits in Winter Wheat Under Different Ecological Environments[J].Scientia Agricultura Sinica,2009,42(2):398-407. | |
| Authors: | WANG Rui-xia ZHANG Xiu-ying WU Ling WANG Rui HAI Lin YOU Guang-xia YAN Chang-sheng XIAO Shi-he |
| Institution: | Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement |
| Abstract: | 【Objective】 QTL associated with grain size and related traits was identified and the phenotypic effects of them were estimated. The stability of QTL across different environments were detected. 【Method】 Using a set of 142 recombinant inbred lines (RILs) derived from Chinese winter wheat varieties Yu8679 and Heshangmai, five agronomic traits of grain length (GL), grain width (GW), grain thickness (GT), grain volume (GV) and 1000-grain weight (TGW) were evaluated in four different ecological environments of Beijing (2006 &;2007), Hefei (2007) and Chengdu (2007), respectively. Based on 142 RILs, a genetic map comprising 170 SSR markers and two EST markers (Tx23-24 and Tx37-38) was generated. According to the genetic map and phenotypic data, quantitative trait loci (QTL) were located for these agronomic traits using the composite interval mapping method. 【Result】 A total of 93 QTL involving all wheat chromosomes except 1D and 6A were identified for these five traits over four environments. Among them, 17, 16, 18, 21 and 21 QTL for GL, GW, GT, GV and TGW were identified, respectively. In addition, 18 genomic regions with plenty of QTL were detected on 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 5D, 6A, 6D, 7B and 7D in this study. 【Conclusion】In the present study, 93 QTL for grain size and related traits were detected, which will provide useful information for molecular assistant selection (MAS) in wheat genetic improvement. |
| Keywords: | QTL |
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