| 草菇蛋白酶的分离与纯化 |
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| 引用本文： | 陈明杰,陈恩.草菇蛋白酶的分离与纯化[J].食用菌学报,1999,6(4):46-48. |
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| 作者姓名： | 陈明杰 陈恩 |
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| 作者单位： | 南京农业大学微生物系，农业部食用菌遗传育种重点开放实验室，农业部食用菌遗传育种重点开放实验室，农业部食用菌遗传育种重点开放实验室，农业部食用菌遗传育种重点开放实验室，农业部食用菌遗传育种重点开放实验室 南京 210095，上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201106，上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201106，上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201106，上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201106，上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201106 |
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| 摘 要： | 草菇是我国食用菌主要栽培品种之一,属于典型的高温真菌,低温将诱导草菇细胞内的蛋白质降解,导致草菇菌丝自溶、死亡。蛋白酶在草菇低温自溶过程中起了重要作用。草菇子实体经破碎、(NH_4)_2SO_4分步沉淀、Sephadex G-150凝胶过滤,获得草菇蛋白酶样品,为草菇的低温自溶与蛋白酶之间关系的研究奠定了基础。
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| 关 键 词： | 草菇 蛋白酶 自溶 |
Isolation and Purification of Proteinase from Volvariella volvacea |
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| Chen Mingjie.Isolation and Purification of Proteinase from Volvariella volvacea[J].Acta Edulis Fungi,1999,6(4):46-48. |
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| Authors: | Chen Mingjie |
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| Abstract: | Votvariella volvacea, a typical kind of thermophilous cultivation mushroom, is one of the major cultivation mushrooms in China. Low temperature can induce protein degradation in V. volvacea cell resulting in mycelium autolysis. It was proved in previous experiments that proteinase played an important role during the autol-ysis of V. volvacea, and that V. volvacea proteinase could be isolated and purified by breaking the fruitbody, through fractional precipitation with ammonium sulfate and Sephadex G- 150 gel filtration which helped to understand the relationship between proteinase and autolysis in V. volvacea. |
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| Keywords: | Volvariella volvacea Proteinase Autolysis |
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