| GPxs家族基因在山羊不同组织和睾丸不同发育时期的表达特性研究 |
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| 引用本文： | 张建新,王茜,荀文娟,任有蛇,岳文斌,张春香.GPxs家族基因在山羊不同组织和睾丸不同发育时期的表达特性研究[J].畜牧兽医学报,2011,42(5). |
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| 作者姓名： | 张建新 王茜 荀文娟 任有蛇 岳文斌 张春香 |
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| 作者单位： | 山西农业大学动物科技学院,太谷,030801 |
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| 基金项目： | 国家自然科学基金资助项目，山西农业大学博士基金 |
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| 摘 要： | 为了研究谷胱甘肽过氧化酶家族(GPxs)在山羊不同组织表达的特异性及在睾丸不同发育时期的表达特性.采用SYBR Green荧光定量PCR的方法对太行青山羊公羊各组织和不同发育时期睾丸GPxs mRNA的表达进行了定量分析.结果表明:GPxl、GPx3、GPx4和GPx7在各组织广泛表达,其表达量由高到低的顺序:GPx1是肝脏＞肺脏＞心脏＞睾丸、脾脏＞肾脏＞背最长肌,肝脏GPxl mRNA表达量是肌肉组织的6.7倍;GPx3是肾脏＞心脏＞肝脏、睾丸＞脾脏、肺脏、背最长肌,肾脏GPx3 mRNA表达量分别是肝脏和肺脏表达量的12和24倍;GPx4是睾丸＞心脏＞肾脏、肝脏、肺脏＞脾脏、背最长肌,睾丸GPx4 mRNA表达量分别是肝脏和肌肉的21和137倍;GPx7是肝脏＞脾脏、肺脏、背最长肌＞心脏＞肾脏、睾丸.GPx5和GPx6仅在睾丸和附睾中表达,且GPx5在附睾头中的表达是睾丸的114倍;睾丸中GPx3表达量随日龄增加而降低,GPxl和GPx4表达量随日龄增加而增加,GPx5在90 d开始表达且直接到达高峰平台,GPx6和GPx7在60 d开始表达,90 d到高峰平台,而后其表达量降低.山羊GPxs mRNA表达具有明显的组织特异性,山羊睾丸中GPxs mRNA表达具有时间依赖性.
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| 关 键 词： | 山羊 GPxs mRNA 组织分布 睾丸 表达特性 |
The Expression of Glutathione Peroxidases Family Genes in Different Tissues and Testes at Different Stages of Male Goats |
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| ZHANG Jian-xin,WANG Qian,XUN Wen-juan,REN You-she,YUE Wen-bin,ZHANG Chun-xiang.The Expression of Glutathione Peroxidases Family Genes in Different Tissues and Testes at Different Stages of Male Goats[J].Acta Veterinaria et Zootechnica Sinica,2011,42(5). |
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| Authors: | ZHANG Jian-xin WANG Qian XUN Wen-juan REN You-she YUE Wen-bin ZHANG Chun-xiang |
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| Institution: | ZHANG Jian-xin,WANG Qian,XUN Wen-juan,REN You-she,YUE Wen-bin,ZHANG Chun-xiang(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China) |
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| Abstract: | The objectives of this study were to evaluate the tissue-specific expression of GPxs in various tissues of the male goat and investigate the expression of GPxs in testes at different stages of male goats.Total RNA was isolated from various tissues,and mRNA expression was detected by real-time fluorescent quantitative PCR.The quantitative real-time PCR results showed that the order of GPx1 mRNA expression level was liver > lung > heart >testes,spleen > kidney > Longissimus dorsi(LD),the expression of GPx1 in... |
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| Keywords: | goat GPxs mRNA tissue distribution testis expression |
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