| 传染性法氏囊病病毒超强毒感染SPF鸡免疫器官中CD4+和CD8+T淋巴细胞动态分布 |
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| 引用本文： | 刘爵,韦莉,姚炜光,张方亮,周蛟,佘锐萍,刘伟,刘尚高,王平.传染性法氏囊病病毒超强毒感染SPF鸡免疫器官中CD4+和CD8+T淋巴细胞动态分布[J].中国兽医学报,2002,22(1):15-18. |
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| 作者姓名： | 刘爵 韦莉 姚炜光 张方亮 周蛟 佘锐萍 刘伟 刘尚高 王平 |
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| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所,北京,100089
2. 中国农业大学动物医学院,北京,100094 |
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| 基金项目： | 国家攀登计划资助项目(85-44-14) |
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| 摘 要： | 利用免疫组织化学染色对传染性法氏囊病病毒（IBDV）超强毒LX株感染SPF鸡免疫器官中CD4^ 和CD8^ T淋巴细胞的动态分布进行了研究。超强毒LX株接种2周龄SPF雏鸡，在其法氏囊、脾脏、胸腺、盲肠扁桃体、骨髓和哈氏腺中均可检出IBDV抗原的存在和CD4^ 与CD8^ T淋巴细胞的数量改变。在法氏囊中，CD4^ 淋巴细胞主要存在于淋巴滤泡间隙和滤泡皮质，而CD8^ T淋巴细胞则丰在于整个淋巴滤泡和滤泡间隙，并且CD8^ T淋巴细胞数量明显多于CD4^ T淋巴细胞，在接种后14d仍未见CD4^ 和CD8^ T淋巴细胞数量减少。脾脏中CD4^ T淋巴细胞主要存在于外周小动脉淋巴鞘或散在，而CD8^ T淋巴细胞则多存在于外周小动脉淋巴鞘和红髓。接种后胸腺中CD4^ 和CD8^ T淋巴细胞在皮质中减少，但在髓质增多，尤其是CD8^ T淋巴细胞数明显多于CD4^＋T淋巴细胞。盲肠扁桃体中CD4^ 和CD8^ T淋巴细胞主要存在于发生中心，尤其是CD8^ T淋巴细胞数比CD4^ T淋巴细胞明显多。骨髓和哈氏腺中也可见CD4^ 和CD8^ T淋巴细胞，而且CD8^ T淋巴细胞更多。在这些淋巴器官中，病毒损伤部位出现CD4^ 和CD8^ T淋巴细胞的迁入聚集，表明T淋巴细胞可能参与IBDV超强毒的免疫致病过程。
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| 关 键 词： | 传染性法氏囊病病毒超强毒 免疫器官 CD4^＋T淋巴细胞 CD8^＋T淋巴细胞 动态分布 鸡 |
| 文章编号： | 1005-4545(2002)01-0015-04 |
| 修稿时间： | 2000年12月28 |
Kinetic Distributions of Subsets CD4+ and CD8+ T Lymphocytes in Various Im mune Organs of SPF Chicks with Inoculation of very Virulent Infectious Bursal Disease Virus |
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| LIU Jue ,WEI Li ,YAO Wei guang ,ZHANG Fang liang ,ZHOU Jiao ,SHE Rui ping ,LIU Wei ,LIU Shang gao ,WANG Ping.Kinetic Distributions of Subsets CD4+ and CD8+ T Lymphocytes in Various Im mune Organs of SPF Chicks with Inoculation of very Virulent Infectious Bursal Disease Virus[J].Chinese Journal of Veterinary Science,2002,22(1):15-18. |
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| Authors: | LIU Jue WEI Li YAO Wei guang ZHANG Fang liang ZHOU Jiao SHE Rui ping LIU Wei LIU Shang gao WANG Ping |
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| Institution: | LIU Jue 1,WEI Li 1,YAO Wei guang 1,ZHANG Fang liang 1,ZHOU Jiao 1,SHE Rui ping 2,LIU Wei 2,LIU Shang gao 2,WANG Ping 1 |
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| Abstract: | Immunohistochemical staining was used to detect kinetic alterations of subsets CD4 + and CD8 + T lymphocytes and distribution of infectious bursal disease virus(IBDV) with very virulent IBDV(vvIBDV) strain LX.The IBD viral antigens were observed in cytoplasm located in bursae,spleen,thymus,cecal tonsils,bone marrow,and Harderian gland,and numerous CD4 + and CD8 + T lymphocytes observed in lesion areas located in these lymphoid tissues postinoculation(PI).In bursae,the number of CD4 + T cells was always lower than the number of CD8 + T cells,and CD4 + cells were mostly found in clusters in the interfollicular tissue and cortex,whereas CD8 + cells were more scattered and mostly infiltrated the cortex and medullae or their remnants.And obvious decreases in the number of CD4 + and CD8 + T cells were not still observed on the fourteenth day PI.In spleen,CD4 + T cells were mainly found in the periarteriolar lymphoid sheaths or scattered,whereas CD8 + T cells tended to cluster in the periarteriolar lymphoid sheaths and red pulp.In thymus,decreases in the number of CD4 + and CD8 + T cells in the cortex were evident,whereas these cells increased in the medulla,and CD8 + cells were more prevalent than CD4 + cells.CD4 + and CD8 + T cells were present in the germinal centers of cecal tonsils PI,but the number of CD8 + cells was obviously higher than the number of CD4 + cells.CD4 + and CD8 + T cells were also detected in the bone marrow and Harderian gland PI.This results showed that CD4 + and CD8 + T lymphocytes flowed into and located in lesion areas of various immune organs after inoculation of vvIBDV strain LX, indicating that T lymphocytes may play a role in the immunopathology of very virulent infectious bursal disease virus infection. |
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| Keywords: | very virulent infectious bursal disease virus immune organs CD4 + T lymphocytes CD8 + T lymphocytes kinetic distribution |
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