Influence of variety, seed position and seed source on screening for coleoptile length in bread wheat (Triticum aestivum L.) |
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| Authors: | TL Botwright GJ Rebetzke AG Condon RA Richards |
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| Institution: | (1) Biology Institute of Shanxi, Taiyuan, Shanxi Province, China, 030006;(2) Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada, K1A 0C6;(3) Department of Crop Science, University of Guelph, Guelph, Ontario, Canada, N1G 2W1;(4) Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food Canada, Kentville, Nova Scotia, Canada, B4N 5E3 |
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| Abstract: | A study was conducted to evaluate the usefulness of somaclonal variation as a means to obtain powdery mildew resistance in
the background of an agronomically elite, high-yielding barley (Hordeum vulgare L.) cultivar. A total of 170 Ro-derived lines were regenerated from embryo-induced callus of the barley cultivar Léger. Forty-five lines were selected and
evaluated in replicated field plots at two locations in Eastern Canada. In comparison with Léger, one of the 45 lines was
higher yielding, one produced a greater test weight, two had a greater seed weight, and one was shorter in plant height. Three
lines were found to segregate for resistance to powdery mildew (Erysiphe graminis DC ex Merat f. sp. hordei EM). Many of the single-plant selections from the three resistant lines showed resistance to powdery mildew under field conditions
for two years. Three lines were eventually promoted to the official registration tests in Ontario. One of the three lines
was subsequently registered as a new cultivar (AC Malone) in Canada. To our knowledge, AC Malone is the world's first barley
cultivar selected from somaclonal variation. The results of this study demonstrate that it is possible to obtain high- yielding
or disease-resistant lines from callus culture in barley.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | barley high yield Hordeum vulgare powdery mildew somaclonal variation |
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