Chitinolytic <Emphasis Type="Italic">Streptomyces griseorubens</Emphasis> E44G enhances the biocontrol efficacy against <Emphasis Type="Italic">Fusarium</Emphasis> wilt disease of tomato |
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| Authors: | Younes M Rashad Abdulaziz A Al-Askar Khalid M Ghoneem Wesam I A Saber Elsayed E Hafez |
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| Institution: | 1.Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute,City of Scientific Research and Technological Applications,Alexandria,Egypt;2.Botany and Microbiology Department, Faculty of Science,King Saud University,Riyadh,Saudi Arabia;3.Department of Seed Pathology Research, Plant Pathology Research Institute,Agricultural Research Center,Giza,Egypt;4.Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute,Agricultural Research Center,Giza,Egypt |
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| Abstract: | Streptomyces griseorubens E44G is a chitinolytic bacterium isolated from cultivated soil in Saudi Arabia (a hot, arid climatic region). In vitro, antifungal potential of S. griseorubens E44G was assessed against the phytopathogenic fungus, Fusarium oxysporum f. sp. lycopersici (the causative agent of the Fusarium wilt disease of tomato). An inhibition zone of 24 mm was recorded. The chitinolytic activity of S. griseorubens E44G was proved when the colloidal chitin agar plate method was used. A thermostable chitinase enzyme of 45 kDa molecular weight was purified using gel filtration chromatography. The optimum activity was obtained at 60 °C and pH 5.5. The purified enzyme has shown a very pronounced activity against the phytopathogenic fungus, F. oxysporum. The molecular characterization of the chitinase gene indicated that it consists of 1218 bp encoding 407 amino acids. The phylogentic analysis based on the nucleotide DNA sequence and the deduced amino acids sequence showed high similarity percentages with other chitinases isolated from different Streptomyces species. In the field evaluation, application of both S. griseorubens E44G treatments significantly increased all tested growth and yield parameters and decreased the disease severity compared with the infected-untreated tomato plants suggesting potential as a biocontrol agent. |
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