| 高效氨氮降解菌的筛选·鉴定及降解能力测定 |
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| 引用本文： | 朱伟,李娜.高效氨氮降解菌的筛选·鉴定及降解能力测定[J].安徽农业科学,2008,36(22). |
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| 作者姓名： | 朱伟 李娜 |
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| 作者单位： | 西南大学资源环境学院,重庆,400716 |
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| 摘 要： | 目的]研究微生物降解氨氮的能力,为解决城市生活污水氨氮污染现象提供参考。方法]在以(NH4)2SO4为唯一氮源的培养基中,从生活污水处理污泥中分离、筛选氨氮降解菌株,并运用生物量测试其最适生长条件并进行鉴定,研究在最适条件下的降解能力。结果]分离、筛选出1株高效氨氮降解菌株DX3,经形态学和生理特性鉴定为假单胞菌属(Pseudomonas)。通过生物量测试得出菌株最适生长条件为30℃,摇床转速为110 r/min,pH值8.0,接种量1.0%。在最适生长条件下,DX3对氨氮降解能力显著,当初始氨氮浓度为45mg/L时,24 h降解率达98.73%。结论]该微生物降解污水中氨氮能力显著,可用于生活污水中的氨氮治理。
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| 关 键 词： | 城市生活污水 氨氮降解菌 生物降解 |
Screening and Identification on High Effective Microorganisms Degrading Ammonia-nitrogen and Determination on Its Degradation Ability |
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| ZHU Wei et al.Screening and Identification on High Effective Microorganisms Degrading Ammonia-nitrogen and Determination on Its Degradation Ability[J].Journal of Anhui Agricultural Sciences,2008,36(22). |
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| Authors: | ZHU Wei |
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| Abstract: | Objective] The research aimed to study the microbiological degradation ability for ammonia-nitrogen,providing the reference for solving the problem of ammonia-nitrogen pollution in urban sewage.Method] The high effective microorganism was isolated from urban sewage sludge,then utilized the biomass to test the optimal conditions of growth and identify its species,finally studied the ability of degradation under the optimal conditions.Result] A high effective microorganism named DX3 to degrade ammonia-nitrogen was obtained.Through morphological observation and character studies of physiology and biochemistry,the strain DX3 was identified as Pseudomonas.By tests in biomass,it was determined that the condition of growth of the strain was 30 ℃,with shaker rotary speed 110 r/min,and the initial pH value was 8.0,and inoculum amount 1.0%.Under these conditions for optimum growth,the degradation rate experimental of ammonia-nitrogen was carried out.The results indicated that the ability of DX3 for degrading ammonia-nitrogen was marked.The ammonia-nitrogen could be degraded 98.73% when the initial concentration at about 45 mg/L.Conclusion] The ability of DX3 for degrading ammonia-nitrogen was marked,and it could be treated to control the problem of ammonia-nitrogen pollution in urban sewage. |
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| Keywords: | Urban sewage Degrading ammonia-nitrogen bacteria Biological degrading |
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