An apparatus for collecting total conidia of Blumeria graminis f.sp. hordei from leaf colonies using electrostatic attraction |
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| Authors: | N Moriura Y Matsuda W Oichi S Nakashima T Hirai T Nonomura K Kakutani S Kusakari K Higashi H Toyoda |
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| Institution: | Laboratory of Plant Protection and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505;;Pharmaceutical Research and Technology Institute, Kinki University, Osaka 577-8502;;Agricultural, Food and Environmental Sciences Research Center of Osaka Prefecture, Osaka 583-0862;;and Tsukuba Research Laboratory, Toagosei Co. Ltd, Ibaraki 300-2611, Japan |
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| Abstract: | Conidia from living conidiophores of barley powdery mildew ( Blumeria graminis f.sp. hordei ) on host leaves were collected consecutively using an electrostatic spore collector. The collector consisted of an electrical conductor plate linked to an electrostatic voltage generator and insulator plates placed abreast on a timed conveyer. The conductor plate was negatively charged by the potential supplied from the voltage generator. The negatively charged conductor plate caused dielectric polarization of the insulator plate, and the surface charge on the insulator plate attracted mature conidia abstricted from conidiophores on colonies growing on leaves placed 2 cm from the insulator plate. The surface charge on the insulator plate was proportional to the voltage applied to the conductor plate. Under optimized conditions, abstricted conidia were attracted to the electrostatically activated insulator plates without any detriment to their survival. During a colony's life span of c . 460 h, conidia were released throughout the day and c . 12 × 104 conidia were collected during the lifetime of the colony. This is the first report on the direct quantification of progeny conidia produced by powdery mildew infecting host leaves. |
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| Keywords: | barley powdery mildew conductor dielectric polarization electrostatic induction insulator spore collector |
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