| 大叶藻黄酮对酒精性肝损伤的保护作用 |
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| 引用本文： | 李静,张朝辉,段筱杉,应锐.大叶藻黄酮对酒精性肝损伤的保护作用[J].水产学报,2016,40(5):799-806. |
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| 作者姓名： | 李静 张朝辉 段筱杉 应锐 |
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| 作者单位： | 中国海洋大学食品科学与工程学院,山东青岛,266003 |
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| 基金项目： | 国家自然科学基金(31272705) |
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| 摘 要： | 为了深入挖掘大叶藻黄酮(ZF)的生物活性,提高大叶藻的利用价值,本实验研究了ZF对酒精性肝损伤的保护作用;实验采用纤维素酶-超声波辅助复合浸提法提取大叶藻中的天然活性物质黄酮类化合物,并采用聚酰胺树脂柱层析法对其进行纯化;研究ZF的体外抗氧化能力;将60只ICR小鼠随机分成6组,通过建立酒精性肝损伤模型,研究ZF对肝损伤的保护作用;结果显示,经纯化后ZF的含量达到80%。体外实验表明ZF对DPPH自由基和羟基自由基有清除能力,具有一定的抗氧化活性;体内实验表明ZF对酒精性肝损伤小鼠的抗氧化能力、脂质代谢能力以及乙醇代谢能力均有影响。ZF各剂量组与模型组相比,血清ALT、AST和γ-GT活性显著降低,肝组织MDA含量显著降低,乙醇代谢酶活性显著提高;ZF中、高剂量组小鼠肝组织GSH-Px和SOD活性显著提高,血脂浓度显著降低。长期过量饮酒可导致小鼠肝脏严重损伤,ZF可以改善小鼠肝组织损伤情况,对酒精性肝损伤起到保护作用,其机制可能与增强机体抗氧化能力、调节脂质代谢和乙醇代谢能力有关。
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| 关 键 词： | 大叶藻 黄酮 抗氧化 酒精性肝损伤 |
| 收稿时间： | 2015/9/14 0:00:00 |
| 修稿时间： | 2015/11/21 0:00:00 |
Protective effect of flavonoids from Zostera marina against chronic alcohol hepatic injury in mice |
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| LI Jing,ZHANG Zhaohui,DUAN Xiaoshan and YING Rui.Protective effect of flavonoids from Zostera marina against chronic alcohol hepatic injury in mice[J].Journal of Fisheries of China,2016,40(5):799-806. |
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| Authors: | LI Jing ZHANG Zhaohui DUAN Xiaoshan and YING Rui |
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| Institution: | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China and College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China |
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| Abstract: | The aim of this study was to investigate the protective effect and its mechanism in the context of antioxidant of flavonoids from Zostera marina (ZF) on chronic alcohol hepatic injury in mice.This study would provide scientific basis for the prevention of chronic alcohol hepatic injury.ZF were extracted by the combination of cellulose and ultrasonic wave assisted and purified by polyamide resin column chromatography.The scavenging DPPH and hydroxyl free radicals abilities of ZF were studied.To establish chronic alcohol hepatic injury model,60 ICR mice were randomly divided into 6 groups (10 mice per group):normal group (C),model group (M),positive group (P),FL group treated with ZF at dose of 40 mg/kg·d,FM group treated with ZF at dose of 80 mg/kg·d and FH group treated with ZF at dose of 160 mg/kg·d.Mice in normal group were treated with distilled water by gavage,and mice in other groups were treated with 50％ alcohol by gavage.60 min later,mice in normal group and model group were given distilled water,positive group mice were given bifendate and other group mice were given ZF of different doses.After 6 weeks of treatment,all mice were killed and blood samples were taken to measure the activities of ALT,AST,γ-GT and the contents of TC and TG.And liver was taken to detect the activities of GSH-Px,SOD,ADH,ALDH and the content of MDA.The ZF flavonoids content reached 80％ after purification.The antioxidant activity of ZF was evaluated by in vitro experiments.In vivo experiments,the liver index of model group was significantly increased,while the liver index of ZF treated groups was significantly lower than that of model group.These results suggest that ZF have some protective effect against alcohol hepatic injury.The activities of ALT,AST,γ-GT and lipid levels in serum and the MDA content of liver in model group significantly increased;the activities of GSH-Px,SOD and ethanol metabolic enzymes of liver in model group evidently decreased,indicating that chronic consumption of alcohol resulted in a serious hepatic injury in mice,and the model of chronic alcohol hepatic injury of mice succeeded.Compared with model group,ZF groups significantly reduced the activities of ALT,AST,γ-GT and lipid levels in serum,the content of MDA in liver increased the activities of GSH-Px,SOD and ethanol metabolic enzymes.The results show that ZF protected mice against chronic alcohol hepatic injury by increasing antioxidant capacity,regulating lipid metabolism and alcohol metabolism.Long-term excessive drinking can lead to hepatic injury;ZF have protective effects against chronic alcohol hepatic injury,and the mechanism is related to antioxidant capacity,lipid metabolism and alcohol metabolism. |
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| Keywords: | Zostera marina flavonoids antioxidant alcoholic hepatic injury |
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