| 绞股蓝提取物抗氧化活性评价 |
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| 引用本文： | 景永帅,吴兰芳,张振东,杨娟.绞股蓝提取物抗氧化活性评价[J].贵州大学学报(农业与生物科学版),2010,29(1):43-47. |
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| 作者姓名： | 景永帅 吴兰芳 张振东 杨娟 |
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| 作者单位： | 1. 贵州大学,生命科学学院,贵州,贵阳,550025;中国科学院天然产物化学重点实验室,贵州,贵阳,550002
2. 中国科学院天然产物化学重点实验室,贵州,贵阳,550002 |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展规划(973计划) |
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| 摘 要： | 采用水杨酸法、二苯代苦味基肼自由基(DPPH.)法和Fe3+还原能力试验3种方法,对绞股蓝不同极性溶剂提取物进行体外抗氧化活性评价,以乙醇、石油醚、氯仿、乙酸乙酯、正丁醇和水为样品提取剂,以Vc作阳性对照。结果表明:除石油醚提取物外,绞股蓝各提取物都表现出较强的清除羟基自由基活性。其中粗多糖对羟基自由基的清除能力最强,半清除率浓度为0.203 mg/mL;绞股蓝各提取物对DPPH自由基也有很强的清除作用,以乙酸乙酯萃取物清除能力为最强,半清除率浓度为0.008 mg/mL。同时,以上提取物还对Fe3+具有很强的还原能力,乙酸乙酯萃取物的还原力优于对照品Vc。
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| 关 键 词： | 绞股蓝 抗氧化 自由基 还原力 |
Evaluation on Antioxidant Activities of Extracts from Gynostemma pentaphyllum |
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| JING Yong-shuai,WU Lan-fang,ZHANG Zhen-dong,YANG Juan.Evaluation on Antioxidant Activities of Extracts from Gynostemma pentaphyllum[J].Journal of Mountain Agriculture & Biology,2010,29(1):43-47. |
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| Authors: | JING Yong-shuai WU Lan-fang ZHANG Zhen-dong YANG Juan |
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| Institution: | 1. College of Life Science, Guizhou University, Guiyang 550025, China ; 2. Key Laboratory of Chemistry for Natural Products of Chinese Academy of Sciences, Guiyang 550002, Guizhou Province, China) |
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| Abstract: | Three methods, including salicylic acid, DPPH （2,2-diphenyl-1-picrylhydrazyl） and deoxidization Fe3 ＋, were used to evaluate the in vitro antioxidant activities of extracts from Gynostemma pentaphyUum using ethanol, petroleum ether, chloroform, ethyl acetate, n-butanol or water, and Vc was employed as a control. The results showed that with the exception of petroleum ether extract, the extracts had good scavenging activities for hydroxyl radical （·OH）, especially crude polysaccharide, which demonstrated the highest activitie and its half elimination ratio （ IC50 ） was 0. 203 mg/mL. The extracts possessed strong scavenging activities on DPPH radical, the effect of ethyl acetate extracts was the strongest and its IC50 value was 0. 008 mg/mL. Additionally, the obtained extracts had stronger deoxidize Fe3＋ ability, the activity of the ethyl acetate extracts was somewhat stronger than that of Vc. |
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| Keywords: | Gynostemma pentaphyllum Antioxidetive activity Free radical Deoxidization |
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