| 超声波协同纤维素酶提取骏枣多糖工艺优化 |
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| 引用本文： | 王俊钢,刘成江,吴洪斌,李宇辉,赵志永,金新文.超声波协同纤维素酶提取骏枣多糖工艺优化[J].广东农业科学,2012,39(1):90-93. |
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| 作者姓名： | 王俊钢 刘成江 吴洪斌 李宇辉 赵志永 金新文 |
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| 作者单位： | 新疆农垦科学院 |
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| 基金项目： | 新疆生产建设兵团科技攻关项目(2010GG61) |
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| 摘 要： | 在单因素试验的基础上,选择纤维素酶添加量3 500 U/g(酶活200 U/mg以上),浸提时间40 min,利用Box-Behnken的中心组合设计及响应面法(RSM)探讨了超声波功率、液料比、pH值和温度等因素的优化组合提取骏枣多糖,通过建立二次回归模型,确定其最佳提取工艺条件为:超声波功率330 W、液料比18、pH值6.7、温度54℃。在此工艺条件下,骏枣多糖的提取量为7.25 g。结果表明,超声波协同纤维素酶法是提高骏枣多糖得率的有效途径之一。
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| 关 键 词： | 骏枣 多糖 纤维素酶 超声波 提取 |
Optimization on cellulase and ultrasonic wave assist extraction technology of Zizyphus jujuba cv.Junzao polysaccharides |
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| WANG Jun-gang,LIU Cheng-jiang,WU Hong-bin,LI Yu-hui,ZHAO Zhi-yong,JIN Xin-wen.Optimization on cellulase and ultrasonic wave assist extraction technology of Zizyphus jujuba cv.Junzao polysaccharides[J].Guangdong Agricultural Sciences,2012,39(1):90-93. |
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| Authors: | WANG Jun-gang LIU Cheng-jiang WU Hong-bin LI Yu-hui ZHAO Zhi-yong JIN Xin-wen |
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| Institution: | (Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China) |
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| Abstract: | On the basis of single factor experiment,confirm cellulase amount 3 500 U/g(enzyme activity was more than 200 U/mg) and extraction time 40 min,the optimization was applied to discuss some factors including ultrasound power,the ratio of solid to liquid,pH value,temperature with Box-Behnken center-united design and themethod of response surface analysis(RSM).Through establishing the second regression model,the optimal parameters were conformed as follows,ultrasound power 330 W,ratio of solid to liquid 1∶18,pH value 6.7,temperature 54℃.Then the value of Zizyphus jujuba cv.polysaccharides was 7.25 g.These results suggested that cellulase assisted by ultrasonic wave extraction technology was one of the effective ways to improve the yield of Z.jujuba cv.polysaccharides. |
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| Keywords: | Zizyphus jujuba cv Junzao polysaccharides cellulase ultrasonic extraction |
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