The Recombinant Nonstructural Polyprotein NS1 of Porcine Parvovirus (PPV) as Diagnostic Antigen in ELISA to Differentiate Infected from Vaccinated Pigs |
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| Authors: | L Qing J Lv H Li Y Tan H Hao Z Chen J Zhao H Chen |
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| Institution: | (1) Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China |
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| Abstract: | To differentiate pigs infected with porcine parvovirus (PPV) from those vaccinated with inactivated whole-virus vaccine, an
enzyme-linked immunosorbent assay (ELISA) based on detection of a nonstructural polyprotein 1 (NS1) was developed. A threshold
of 0.23 optical density units was established and the assayhad high specificity (100), sensitivity (88), accuracy (90) and
positive predictive value (100) using haemagglutination inhibition as the standard method. A reproducibility test revealed
that the coefficients of variation of sera within-plates and between-run were less than 10%. The assayshowed no cross-reactivitywith
antibodies to porcine reproductive respiratorysyndrome virus, pseudorabies virus, foot and mouth disease virus, Actinobacillus pleuropneumoniae, Toxoplasma or Chlamydia. Sera obtained from pigs infected with PPV reacted with recombinant NS1 protein in the ELISA. Sera from pigs vaccinated with
inactivated whole virus did not recognize this protein in the ELISA. In contrast, antibodies against PPV whole virus were
present in both PPV-infected and vaccinated animals. Serum conversion against NS1 was first detected 10 days after infection
and NS1-specific antibodies were detectable up to half a year post infection. In conclusion, the PPV-NS1 ELISA can differentiate
PPV-infected versus inactivated PPV-vaccinated pigs and could be applied in disease diagnosis and surveillance. |
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| Keywords: | differentiation diagnostic method ELISA nonstructural protein NS1 porcine parvovirus (PPV) |
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