| 云南越桔花粉活力测定与花粉保存 |
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| 引用本文： | 杨洪涛,李燕,杨燕林,和志娇,王朝文,黄杏娥,和加卫.云南越桔花粉活力测定与花粉保存[J].江西农业学报,2014(6):56-58. |
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| 作者姓名： | 杨洪涛 李燕 杨燕林 和志娇 王朝文 黄杏娥 和加卫 |
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| 作者单位： | 云南省农业科学院高山经济植物研究所,云南丽江674100 |
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| 基金项目： | 农业部公益性行业科技项目（201103037）; 农业部成果转化项目（2012GB2F300414） |
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| 摘 要： | 用不同浓度蔗糖、硼酸对云南越桔花粉进行离体培养后统计其萌发率,结果表明:云南越桔花粉最适宜的离体培养基为15%蔗糖+0.1%硼酸+1%琼脂,花粉萌发最好,萌发率高达78.26%。此外,4℃恒温、避光条件下贮藏了不同时间的花粉,在适宜培养基中的离体培养萌发率试验表明:云南越桔花粉现采的活力最高,花粉的贮藏时间以不超过1个月为宜。
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| 关 键 词： | 云南越桔 花粉 花粉活力 贮藏时间 |
Vitality Determination and Preservation of Pollens in Vaccinium duclouxii |
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| Institution: | YANG Hong - tao, LI Yan, YANG Yan- lin, HE Zhi - jiao, WANG Chao - wen, HUANG Xing - e, HE Jia - wei ( Institute of Alpine Economic Plants, Yunnan Academy of Agricultural Sciences, Lijiang 674100, China) |
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| Abstract: | Different concentrations of sucrose and boric acid were used for the isolated culture of pollens in Vaccinium du-clouxii, and the germination rate of the pollens in vitro was statistically analyzed .The results indicated that the optimum culture me-dium for Vaccinium duclouxii pollens in vitro was 15%sucrose ＋0.1%boric acid ＋1%agar, and the germination rate of the pol-lens in this culture medium was the highest , reaching 78.26%.In addition, the isolated Vaccinium duclouxii pollens which have been preserved under the conditions of constant temperature 4℃and darkness for a different time were cultivated on suitable culture medium , and their germination rate was tested .The experimental results showed that the fresh -collected pollens had the highest vi-tality, and the storage time for the pollens should not be longer than one month . |
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| Keywords: | Vaccinium duclouxii Pollen Vitality Storage time |
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