| 不同激活剂对兔卵母细胞孤雌激活效果的研究 |
| |
| 引用本文： | 王鸽,张君涛,张胜军,魏莉莉,曹书郡,徐秋勤,柳文龙,刘童,张志平.不同激活剂对兔卵母细胞孤雌激活效果的研究[J].江西农业学报,2014(9):90-93. |
| |
| 作者姓名： | 王鸽 张君涛 张胜军 魏莉莉 曹书郡 徐秋勤 柳文龙 刘童 张志平 |
| |
| 作者单位： | 河南农业大学牧医工程学院,河南郑州450002 |
| |
| 基金项目： | 国家自然科学基金（31001096）; 河南农业大学雏鹰农牧大学生创新创业基金 |
| |
| 摘 要： | 目的:探寻一种稳定有效的卵母细胞激活方法,为兔体细胞核移植提供实验基础。方法:兔超排后获得卵母细胞体外成熟22~24 h,经脱颗粒处理后采用不同激活剂和不同处理时间进行激活,体外培养5~7 d,观察胚胎发育与囊胚形成情况。结果:卵母细胞经过离子霉素作用5 min或者乙醇作用7 min均能被激活,但两者卵裂率、囊胚率都较低;乙醇和离子霉素分别与6-DMAP联合激活兔卵母细胞时,离子霉素+6-DMAP处理组卵裂率(73.7%)显著高于乙醇+6-DMAP处理组(38.5%,P<0.05);与离子霉素联合激活时,2.0 mmol/L的6-DMAP处理5.0 h时的卵裂率和囊胚率最高,达75.6%和45.5%。结论:激活方法对兔卵母细胞孤雌激活率的影响很大,不同激活剂的联合应用及激活剂的作用时间对兔卵母细胞的激活存在显著差异。
|
| 关 键 词： | 兔卵母细胞 孤雌激活 离子霉素 乙醇 6-二甲基氨基嘌呤 |
Study on Effect of Different Stimuli on Parthenogenetic Activation of Rabbit Oocytes |
| |
| Institution: | WANG Ge , ZHANG Jun - tao , ZHANG Sheng - jun, WEI Li - li, CAO Shu - jun, XU Qiu - qin, LIU Wen - long, LIU Tong, ZHANG Zhi - ping＊ ＊ (College of Animal and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China) |
| |
| Abstract: | The objective of this study is to seek a stable and effective method for the parthenogenetic activation of rabbit oocytes,and make an experimental foundation for the somatic cell nuclear transfer of rabbit. Cumulus- oocyte complexes were harvested from the ovaries of superovulated rabbits,and were matured in vitro for 22 - 24 h; after removing the cumulus cells,the oocytes were activated by various stimuli for different time. After in vitro culture for 5 - 7 d,the parthenogenetic development of stimulated oocytes was assessed by microscopic observation. The results showed that rabbit oocytes could be activated not only by ionomycin treatment for 5 min,but also by ethanol treatment for 7 min,but their cleavage rate and blastula rate all were relatively low. When6- DMAP combined with ionomycin or ethanol was used to activate rabbit oocytes,the cleavage rate（ 73. 7%） in the treatment of ionomycin ＋ 6- DMAP was significantly（ P 〈0. 05） higher than that（ 38. 5%） in the treatment of ethanol ＋ 6- DMAP; the treatment of ionomycin ＋ 2. 0 mmol /L 6- DMAP for 5. 0 h obtained the highest cleavage rate and blastula rate,being 75. 6% and45. 5% respectively. It was concluded that activation method had great influence on the parthenogenetic activation rate of rabbit oocytes,and there were significant differences in activation effect among different treatments（ combined utilization of different stimuli,various activation time）. |
| |
| Keywords: | Rabbit oocytes Parthenogenetic activation Ionomycin Ethanol 6-DMAP |
| 本文献已被 维普 等数据库收录！ |
|
