| 基于16S rRNA和Cyt b基因序列探讨2种梅童鱼的遗传分化 |
| |
| 引用本文： | 马国强,高天翔,孙典荣.基于16S rRNA和Cyt b基因序列探讨2种梅童鱼的遗传分化[J].南方水产,2010,6(2):13-20. |
| |
| 作者姓名： | 马国强 高天翔 孙典荣 |
| |
| 作者单位： | 1. 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003
2. 中国水产科学研究院南海水产研究所,广东,广州,510300 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)项目(2006AA09Z418);;国家自然科学基金项目(30471329) |
| |
| 摘 要： | 比较分析了棘头梅童鱼（Collichthys lucidus）和黑鳃梅童鱼（C.niveatus）的16SrRNA和Cytb基因片段序列差异及遗传分化程度。在长度为526bp的16SrRNA和379bp的Cytb基因片段的核苷酸序列中，2种间共检测到44处核苷酸替代。分析结果表明，2个基因片段的鸟嘌呤（G）含量较低，在Cytb蛋白质编码基因第三密码子位点上表现尤为明显。基于16SrRNA和Cytb基因片段分析结果显示，2种间平均遗传距离分别为0．012和0．111。构建的系统树显示2种梅童鱼在这2个基因片段上存在显著的遗传分化。根据Cytb基因2％／百万年的进化速率推断，棘头梅童鱼与黑鳃梅童鱼的分化时间约为550万年，发生于上新世（Pliocene）早期。
|
| 关 键 词： | 棘头梅童鱼 黑鳃梅童鱼 16S rRNA Cyt b 遗传分化 |
Discussion of relationship between Collichthys lucidus and C.niveatus based on 16S rRNA and Cyt b gene sequences |
| |
| MA Guoqiang,GAO Tianxiang,SUN Dianrong.Discussion of relationship between Collichthys lucidus and C.niveatus based on 16S rRNA and Cyt b gene sequences[J].South China Fisheries Science,2010,6(2):13-20. |
| |
| Authors: | MA Guoqiang GAO Tianxiang SUN Dianrong |
| |
| Institution: | 1. Key Lab. of Mariculture/a>;Ministry of Education/a>;Ocean University of China/a>;Qingdao 266003/a>;China/a>;2. South China Sea Fisheries Research Institute/a>;Chinese Academy of Fishery Sciences/a>;Guangzhou 510300/a>;China |
| |
| Abstract: | Collichthys lucidus and C.niveatus are commercially important fishery resources in China.Partial nucleotide sequences of mitochondrial DNA 16S rRNA and Cyt b gene fragments were sequenced and analyzed to study the relationship between C.lucidus and C.niveatus.Forty-four nucleotide substitutions were detected among 16S rRNA (526 bp) and Cyt b (379 bp) fragments.Nucleotide composition analysis indicated that the content of guanine (G) was relatively low in the two fragments,especially at the third codon posit... |
| |
| Keywords: | Collichthys lucidu C niveatus 16S rRNA Cyt b genetic differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
