Interspecific hybridization inBrassica juncea × Brassica hirta using embryo rescue |
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| Authors: | D Mohapatra Y P S Bajaj |
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| Institution: | (1) Punjab Agricultural University, 141 004 Ludhiana, India;(2) Present address: NARP, R.R.S., Ranital, 756111 Balasore, Orissa, India |
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| Abstract: | Summary Interspecific hybrids have been obtained in an otherwise incompatible cross betweenBrassica juncea × Brassica hirta through the in vitro culture of hybrid ovules and ovaries. The best response was observed from ovules and ovaries cultured 10–15 and 5–7 days after pollination respectively on a basal medium supplemented with indoleacetic acid, kinetin and casein hydrolysate. In some cases the basal cut end of the ovaries proliferated to form callus and shoots. The in vitro-derived hybrid seeds varied in their colour, size and shape, and the F1 plants in the field showed a large diversity in their morphological traits. The hybrids were sterile, and had an intermediate number of chromosomes (2n=30). |
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| Keywords: | Brassica juncea Brassica hirta white mustard oilseed crops tissue culture interspecific hybridization genetic variation |
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