| 实时荧光PCR快速检测苹果蠹蛾 |
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| 引用本文： | 白桦,魏晓棠,梁炜,尼秀媚,张京宣,宋涛.实时荧光PCR快速检测苹果蠹蛾[J].植物检疫,2011(2). |
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| 作者姓名： | 白桦 魏晓棠 梁炜 尼秀媚 张京宣 宋涛 |
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| 作者单位： | 山东出入境检验检疫局; |
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| 基金项目： | 国家质检总局科研项目(2009IK278) |
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| 摘 要： | 苹果蠹蛾(Cydia pomonella L.)是重要的国际检疫性害虫。本文根据苹果蠹蛾线粒体COI基因序列设计引物和探针,对苹果蠹蛾及其相似种进行实时荧光PCR检测,结果表明,不同虫态的苹果蠹蛾均能够与近似种类区分开来。该技术可快速准确地鉴定苹果蠹蛾,为检疫性害虫苹果蠹蛾的检疫提供了一种新的方法。
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| 关 键 词： | 苹果蠹蛾 实时荧光PCR COI基因 检测 |
Rapid identification of Cydia pomonella L by Real-Time PCR. |
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| Bai Hua,Wei Xiaotang,Liang Wei,Ni Xiumei,Zhang Jingxuan,Song Tao.Rapid identification of Cydia pomonella L by Real-Time PCR.[J].Plant Quarantine,2011(2). |
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| Authors: | Bai Hua Wei Xiaotang Liang Wei Ni Xiumei Zhang Jingxuan Song Tao |
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| Institution: | Bai Hua,Wei Xiaotang,Liang Wei,Ni Xiumei,Zhang Jingxuan,Song Tao(Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China) |
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| Abstract: | Cydia pomonella is an important international quarantine pest.Primers and porbe were designed independing on mtDNA COI sequence.C.pomonella was detected by Real-Time PCR.The result showed that the different stage of C.pomonella individuals can be distinguished from the allied species.So,C.pomonella can be rapid identified by real-time PCR.The technician provided a new method for the quarantine of C.pomonella. |
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| Keywords: | Cydia pomonella Real-Time PCR COI gene detect |
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