Stress mitigating and immunomodulatory effect of dietary pyridoxine in Labeo rohita (Hamilton) fingerlings |
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| Authors: | Mohammad Shahbaz Akhtar Asim Kumar Pal Narottam Prasad Sahu Ciji Alexander Sanjay Kumar Gupta Arup Kumar Choudhary Ashish Kumar Jha Mysore Govindrajan Rajan |
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| Institution: | 1. Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Mumbai, India;2. Laboratory Nuclear Medicine Section, Bhabha Atomic Research Centre, TMH annex, Mumbai, India |
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| Abstract: | A 60‐day experiment was carried out to delineate stress mitigating and immunomodulatory role of dietary pyridoxine (PN) in Labeo rohita fingerlings exposed to endosulfan. Two hundred and seventy fingerlings were randomly distributed into six treatments in triplicates. Five iso‐caloric and iso‐nitrogenous purified diets were prepared with graded levels of pyridoxine. Six treatment groups were T0 (10 mg PN+without endosulfan), T1 (0 mg PN+endosulfan), T2 (10 mg PN+endosulfan), T3 (50 mg PN+endosulfan), T4 (100 mg PN+endosulfan) and T5 (200 mg PN+endosulfan). The role of pyridoxine on stress mitigation and immunomodulation was assessed by biochemical and haemato‐immunological parameters like aspartate aminotransaminase, alanine aminotransaminase, lactate dehydrogenase, malate dehydrogenase, superoxide dismutase and catalase were significantly (P<0.05) lower while acetylcholinesterase was significantly (P<0.05) higher in pyridoxine‐fed groups. Erythrocytes count, haemoglobin content and total serum protein, albumin, globulin, nitroblue tetrazolium and lysozyme activity were significantly (P<0.05) higher while cortisol and blood glucose were decreased significantly (P<0.05) in pyridoxine‐fed groups. Percentage survival after challenge with Aeromonas hydrophila was highest in T0 group. The results obtained in present study indicate that dietary pyridoxine supplementation at 100 mg PN kg?1 diet reduces the endosulfan‐induced stress and triggers immune response in L. rohita fingerlings. |
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| Keywords: | stress pyridoxine cortisol lysozyme AChE Labeo rohita |
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