| 低淀粉饲粮条件下不同瘤胃降解淀粉水平对体外瘤胃发酵的影响 |
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| 引用本文： | 雒国彬,王利军,刘岩,张广宁,孙凯晶,王馨影,张永根.低淀粉饲粮条件下不同瘤胃降解淀粉水平对体外瘤胃发酵的影响[J].动物营养学报,2017,29(7). |
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| 作者姓名： | 雒国彬 王利军 刘岩 张广宁 孙凯晶 王馨影 张永根 |
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| 作者单位： | 东北农业大学动物科学技术学院,哈尔滨,150030 |
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| 基金项目： | 国家奶牛产业技术体系项目 |
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| 摘 要： | 本试验旨在研究玉米作为淀粉来源的低淀粉饲粮条件下不同瘤胃降解淀粉(RDS)水平对体外瘤胃发酵的影响。以3头安装有永久性瘤胃瘘管的健康荷斯坦奶牛作为瘤胃液供体,各组分别以不同RDS水平饲粮作为发酵底物,体外产气法测定培养48 h时产气量和瘤胃发酵参数以及24 h时瘤胃微生物区系变化。结果表明:1)随着饲粮RDS水平的提高,体外培养48 h时产气量、潜在产气部分和产气速率呈线性升高(P0.05),快速发酵部分产气量呈线性下降(P0.05),干物质消失率呈线性升高(P0.05);2)随着饲粮RDS水平的提高,体外培养48 h时发酵液微生物蛋白、乙酸、丙酸、丁酸和总挥发性脂肪酸浓度呈线性升高(P0.05),pH和氨态氮浓度没有显著变化(P0.05);3)随着饲粮RDS水平的提高,体外培养24 h时发酵液中白色瘤胃球菌和嗜淀粉瘤胃杆菌的相对数量呈线性升高(P0.05),黄色瘤胃球菌、琥珀酸丝状杆菌、溶纤维丁酸弧菌、牛链球菌和溶淀粉琥珀酸单胞菌的相对数量没有显著变化(P0.05)。综合考虑,低淀粉饲粮条件下提高RDS水平有利于瘤胃发酵。
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| 关 键 词： | 瘤胃降解淀粉 产气量 瘤胃发酵 微生物区系 |
Effects of Different Ruminally Degradable Starch Levels in Low Starch Diet on in Vitro Ruminal Fermentation |
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| LUO Guobin,WANG Lijun,LIU Yan,ZHANG Guangning,SUN Kaijing,WANG Xinying,ZHANG Yonggen.Effects of Different Ruminally Degradable Starch Levels in Low Starch Diet on in Vitro Ruminal Fermentation[J].Acta Zoonutrimenta Sinica,2017,29(7). |
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| Authors: | LUO Guobin WANG Lijun LIU Yan ZHANG Guangning SUN Kaijing WANG Xinying ZHANG Yonggen |
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| Abstract: | This trial was performed to examine the effects of different ruminally degradable starch levels in the diet with low corn-based starch on in vitro ruminal fermentation.Three Holstein cows fitted with permanent rumen fistulas were used as the donor of rumen fluid.Diets with different ruminally degradable starch levels were used as substrates, and the changes of gas production and ruminal fermentation parameters during 48 h incubation were determined using in vitro gas production method, and the ruminal microflora change during 24 h incubation was also determined.The results showed as follows: 1) with the ruminally degradable starch level increasing, gas production (GP), potential gas production (a+b) and gas production rate constant for "gas production from the slowly soluble fraction (b)" (c) were linearly increased (P<0.05), the gas production from the immediately soluble fraction (a) was linearly decreased (P<0.05), and the dry matter digestibility was linearly increased after 48 h in vitro incubation (P<0.05).2) With the ruminally degradable starch level increasing, fermentation fluid microprotein (MCP), acetate, propionate, butyrate and total volatile fatty acids (TVFA) concentrations were linearly increased after 48 h in vitro incubation (P<0.05), but the pH and ammoni anitrogen concentration were not significantly changed (P>0.05).3) With the ruminally degradable starch level increasing, the relative counts of R.albus and R.amylophilus in fermentation fluid after 24 h in vitro incubation were linearly increased (P<0.05), while the relative counts of R.flavefacien, F.succinogenes, B.fibrisolvens, S.bovis and S.amylolytica had no significant changes (P>0.05).It is concluded that incraesing ruminally degradable starch level can improve ruminal fermentation under the condition of low starch diet. |
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| Keywords: | ruminally degradable starch gas production rumen fermentation microflora |
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