| 茶皂素对奶牛瘤胃原虫区系的影响 |
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| 引用本文： | 赵士萍,严淑红,蒋琦晖,常肖肖,王炳,方洛云,蒋林树,熊本海.茶皂素对奶牛瘤胃原虫区系的影响[J].动物营养学报,2017,29(2). |
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| 作者姓名： | 赵士萍 严淑红 蒋琦晖 常肖肖 王炳 方洛云 蒋林树 熊本海 |
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| 作者单位： | 1. 北京农学院动物科学技术学院,奶牛营养学北京市重点实验室,北京102206;2. 中国农业科学院畜牧研究所,北京,100193 |
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| 基金项目： | "十二五"国家科技支撑项目，"十三五"国家重大科技专项，北京市农业局北京市现代农业产业技术体系奶牛创新团队 |
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| 摘 要： | 本研究旨在研究茶皂素对奶牛瘤胃原虫区系的影响。选取12头健康荷斯坦奶牛体重(550±30)kg,产奶量35 kg/(头·d),胎次2~4],随机分为4组,每组3头,均饲喂基础饲粮,分别于晨饲前灌服0(对照)、20、30、40 g/(头·d)的茶皂素。试验期49 d,其中预试期14 d,正试期35 d,正试期内每7 d于晨饲前1 h用口腔采样器采集瘤胃液。采用聚丙烯酰氨凝胶电泳(DGGE)结合18S r DNA序列分析技术研究瘤胃原虫区系的变化。结果表明:1)添加茶皂素可以选择性地抑制瘤胃中原虫的生长。2)与对照组相比,30、40 g/(头·d)茶皂素组的丰富度指数和香农多样性指数都显著降低(P0.05),优势度指数显著增加(P0.05),但是均一性指数无显著变化(P0.05);茶皂素显著减少了瘤胃中前庭亚纲内毛目的原虫数量。综上所述,饲粮中添加30、40 g/(头·d)茶皂素均可抑制奶牛瘤胃原虫的生长,并且降低瘤胃原虫的多样性。
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| 关 键 词： | 奶牛 原虫区系 茶皂素 |
Effects of Tea Saponin on Rumen Protozoa Flora of Dairy Cows |
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| ZHAO Shiping,YAN Shuhong,JIANG Qihui,CHANG Xiaoxiao,WANG Bing,FANG Luoyun,JIANG Linshu,XIONG Benhai.Effects of Tea Saponin on Rumen Protozoa Flora of Dairy Cows[J].Acta Zoonutrimenta Sinica,2017,29(2). |
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| Authors: | ZHAO Shiping YAN Shuhong JIANG Qihui CHANG Xiaoxiao WANG Bing FANG Luoyun JIANG Linshu XIONG Benhai |
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| Abstract: | The objective of this study was to research the effects of tea saponin on rumen protozoa flora of dairy cows.Twelve healthy Holstein dairy cows body weight was (550 ± 30) kg;milk yield was35 kg/(head · d);2 to 4 parities] were randomly allocated to one of four groups with 3 cows per group.Tea saponin 0 (control), 20, 30 and 40 g/(head · d)] was perfused to cows before morning feeding.The experiment lasted for 49 d with 14 d of adaptation period and 35 d of formal test period.Rumen fluid was collected by mouth sampler at 1 h before morning feeding every 7 d during formal test period.Denaturing gradient gel electrophoresis (DGGE) combined with 18S rDNA sequence analysis technique was used for rumen protozoa flora analysis.The results showed as follows: 1) tea saponin could selectively inhibit protozoa growth.2)Compared with control group, richness index and Shannon index in 30 and 40 g/(head · d) tea saponin groups were significantly reduced (P<0.05), and dominance index was significantly increased (P<0.05), but evenness index was not significantly different among groups (P>0.05);tea saponin could significantly reduce rumen Vestibuliferia Entodiniomorphida protozoa number.In conclusion, dietary supplementation of 30 and40 g/(head · d) tea saponin can inhibit protozoa growth in rumen of dairy cows, and reduce the diversity of rumen protozoa.Chinese Journal of Animal Nutrition, 2017, 29(2) :620-627] |
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| Keywords: | dairy cow protozoa flora tea saponin |
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