| 绿茶粉和绿茶多酚对犬的抗氧化作用 |
| |
| 引用本文： | 李玉,陶焕青,张玉蝶,徐怡钟,郝宁,邹苏萍,冯士彬,王希春,周裔彬,王一君,张梁,吴金节,宛晓春.绿茶粉和绿茶多酚对犬的抗氧化作用[J].动物营养学报,2017,29(10). |
| |
| 作者姓名： | 李玉 陶焕青 张玉蝶 徐怡钟 郝宁 邹苏萍 冯士彬 王希春 周裔彬 王一君 张梁 吴金节 宛晓春 |
| |
| 作者单位： | 1. 安徽农业大学茶树生物学与资源利用国家重点实验室,合肥 230036;安徽农业大学动物科技学院,合肥 230036;2. 安徽农业大学动物科技学院,合肥,230036;3. 安徽农业大学茶树生物学与资源利用国家重点实验室,合肥,230036 |
| |
| 基金项目： | 2016年度安徽省博士后研究人员科研活动经费资助项目，现代农业产业技术体系资助，茶叶化学与健康领军人才团队引进重大示范项目，国家重点研发计划 |
| |
| 摘 要： | 本试验旨在探究绿茶粉及绿茶多酚对犬的抗氧化作用。选用5月龄杂交幼犬15只,随机分为3个组,每组5只。对照组饲喂基础饲粮,绿茶粉组饲喂基础饲粮+1.0%的绿茶粉,绿茶多酚组饲喂基础饲粮+0.25%的绿茶多酚。试验期为84 d。结果表明:与试验开始时相比,试验结束时对照组犬体重增长了162%,绿茶粉组犬体重增长了101%,绿茶多酚组犬体重增长了132%,且绿茶粉组和绿茶多酚组试验结束时犬体重显著低于对照组(P0.05),比对照组分别降低了61%和30%。试验第84天,绿茶粉组及绿茶多酚组犬血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性均显著或极显著低于对照组(P0.05或P0.01),血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性极显著高于对照组(P0.01),血清丙二醛(MDA)含量极显著低于对照组(P0.01),肝脏抗氧化基因谷氨酸半胱氨酸连接酶催化亚基(GCLC)、谷氨酸半胱氨酸连接酶修饰亚基(GCLM)、血红素氧合酶1(HO-1)、过氧化氢酶(CAT)]和Ⅱ相解毒酶基因谷胱甘肽硫转移酶M1(GSTM1)和醌氧化还原酶1(NQO1)]的mRNA相对表达量显著高于对照组(P0.05)。综上所述,在本试验条件下,在犬饲粮中添加1.0%的绿茶粉或0.25%的绿茶多酚均具有保护肝脏、抗氧化、促进Ⅱ相解毒酶基因表达及控制体重的作用。
|
| 关 键 词： | 犬 绿茶粉 绿茶多酚 肝功能 抗氧化基因 Ⅱ相解毒酶基因 |
Antioxidant Effects of Green Tea Powder and Green Tea Polyphenols on Canines |
| |
| LI Yu,TAO Huanqing,ZHANG Yudie,XU Yizhong,HAO Ning,ZOU Suping,FENG Shibin,WANG Xichun,ZHOU Yibin,WANG Yijun,ZHANG Liang,WU Jinjie,WAN Xiaochun.Antioxidant Effects of Green Tea Powder and Green Tea Polyphenols on Canines[J].Acta Zoonutrimenta Sinica,2017,29(10). |
| |
| Authors: | LI Yu TAO Huanqing ZHANG Yudie XU Yizhong HAO Ning ZOU Suping FENG Shibin WANG Xichun ZHOU Yibin WANG Yijun ZHANG Liang WU Jinjie WAN Xiaochun |
| |
| Abstract: | The present study was conducted to investigate the antioxidant effects of green tea powder and green tea polyphenols ( GPTs) on canines. Fifteen 5?month old canines were selected and randomly divided into three groups ( 5 canines in each group) . The canines in control group were fed a basal diet, the canines in green tea power group were fed the basal diet plus 1.00% green tea power, and the canines in GPTs group were fed the basal diet plus 0.25%GPTs. The feeding experiment lasted for 84 days. The results showed as follows: 1) compared with the initial body weight, the final body weight of control group, green tea power group and GTPs group was increased by 162%, 101% and 132%, respectively. The final body weight of green tea power group and GTPs group was significantly lower than that in control group ( P<0.05) , which was decreased by 61% and 30% compare with control group, respectively. On the 84th day of experiment, the activities of ser?um alanine transaminase ( ALT ) , aspartate aminotransferase ( AST ) and lactic dehydrogenase ( LDH ) in green tea power group and GTPs group were significantly or extremely significantly lower than those in control group (P<0.05 or P<0.01), the activities of serum glutathione peroxidase (GSH?Px) and superoxide dis?mutase ( SOD) were extremely significantly higher than those in control group ( P<0.01) , the content of ser?um malondialdehyde ( MDA) was extremely significantly lower than that in control group ( P<0.01) , and the relative mRNA expression levels of antioxidant genes glutamate cysteine ligase catalytic subunit ( GCLC ) , glutamate cysteine ligase subunit modification ( GCLM ) , heme oxygenase 1 ( HO?1 ) and catalase ( CAT ) ] and phase Ⅱ detoxification genes quinone oxidoreductase 1 ( NQO1 ) and glutathione S transferase M1 ( GSTM1) ] in liver were significantly higher than those in control group ( P<0. 01 ) . In summary, adding 1.00% green tea powder or 0.25% GTPs in the diet of canines have the effects of liver protection, antioxidant, activating phase Ⅱ detoxification gene expression and controlling body weight under the condition of the pres?ent study. |
| |
| Keywords: | canines green tea powder green tea polyphenols liver function antioxidant genes phase Ⅱdetoxification genes |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
