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| 猪捷申病毒与猪圆环病毒2型双重PCR检测方法的建立及初步应用 | |
| 引用本文： | 张晓杰,汤德元.猪捷申病毒与猪圆环病毒2型双重PCR检测方法的建立及初步应用[J].中国兽药杂志,2012,46(4):10-13. |
| 作者姓名： | 张晓杰 汤德元 |
| 作者单位： | 1. 贵州大学动物科学学院,贵阳550025／中国兽医药品监察所,北京100081 2. 中国兽医药品监察所,北京,100081 3. 贵州大学动物科学学院,贵阳,550025 |
| 基金项目： | 国家自然科学基金项目（31172341;31172349）;十一五国家科技支撑计划项目（2007BAD86804）;贵州省2011年农业攻关项目[黔科合NY字（2011）3103号] |
| 摘 要： | 为建立可同时检测猪捷申病毒（PTV）与猪圆环病毒2型（PCV2）的双重PCR方法，本研究根据GenBank登录的相关病毒基因序列，选择保守区域设计引物，经过反应条件的优化，建立了可同时检测以上2种病毒的PCR诊断方法，扩增片段长度分别为187bp（PTV）、120bp（PCV2）。通过实验证明该方法具有良好的特异性和较高的敏感性，对PTV、PCV2核酸检测最低浓度分别为2．8×10^-2ng和6．6×10^-3ng。应用该方法对43份临床样品进行检测发现，PTV阳性率为23％，PCV2阳性率为38％，PTV与PCV2共感染率为16％。该方法的成功建立，为快速高效地检测以上2种病毒提供有力手段。 |
| 关 键 词： | 猪捷申病毒 猪圆环病毒2型 双重PCR |
| 收稿时间： | 2011/12/31 0:00:00 |
| 修稿时间： | 2012/1/19 0:00:00 |
Establishment and application of duplex PCR assay for the detection of Porcine teschovirus and Porcine circovirus type 2 |
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| ZHANG Xiao-jie,LIU Ye-bing,TANG De-yuan,LI Chun-yan,ZENG Zhi-yong,ZHU Xiang-bo,ZHOU Li,WANG Feng,GAN Zhen-lei.Establishment and application of duplex PCR assay for the detection of Porcine teschovirus and Porcine circovirus type 2[J].Chinese Journal of Veterinary Drug,2012,46(4):10-13. | |
| Authors: | ZHANG Xiao-jie LIU Ye-bing TANG De-yuan LI Chun-yan ZENG Zhi-yong ZHU Xiang-bo ZHOU Li WANG Feng GAN Zhen-lei |
| Institution: | College of Anima Science Gui Zhou University Guiyang, |
| Abstract: | This experiment was conducted to establish a duplex PCR method whichwas developed for detecting the porcine teschovirus （ PTV ） and porcine circovirus type 2 （ PCV2 ） with a set of specific primers designed according to the published viral genome sequences. The PCR products for each virus were 187 bp （PTV） and 120 bp （PCV2） , with the detection limit of 2.8 ×10^-2 ng and 6.6 × 10^-3 ng, respectively. The method was used to detect 43 clinical samples. Among the samples, PTV percentage was 23%, PCV2 positive percentage was 38% , and co -infection positive percentage was 16%. This duplex PCR assay having been successfully established was proved to be a sensitive, specific method for rapid detection of the two viruses. |
| Keywords: | porcine teschovirus porcine circovirus type 2 duplex PCR |
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