| 团头鲂糖皮质激素受体基因组织表达分布及其在应激中的表达变化 |
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| 引用本文： | 陈凯,习丙文,滕涛,秦婷,潘良坤,谢骏.团头鲂糖皮质激素受体基因组织表达分布及其在应激中的表达变化[J].水产学报,2019,43(4):719-730. |
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| 作者姓名： | 陈凯 习丙文 滕涛 秦婷 潘良坤 谢骏 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心, 农业农村部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;上海海洋大学水产与生命学院, 上海 201306,中国水产科学研究院淡水渔业研究中心, 农业农村部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;淡水水产健康养殖湖北省协同创新中心, 湖北 武汉 430070,南京农业大学无锡渔业学院, 江苏 无锡 214081,中国水产科学研究院淡水渔业研究中心, 农业农村部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081,中国水产科学研究院淡水渔业研究中心, 农业农村部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081,中国水产科学研究院淡水渔业研究中心, 农业农村部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081 |
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| 基金项目： | 现代农业产业技术体系建设专项（CARS-45）；国家自然科学基金（31572662） |
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| 摘 要： | 为了解团头鲂糖皮质激素受体(GR)在应激反应中的调控机制,本研究以皮质醇注射模拟应激事件,采集组织样品,常规检测血糖、血清皮质醇水平;利用荧光定量PCR检测了gr在不同组织中的表达丰度及其参与调控的功能基因的表达变化;并通过常规石蜡切片开展了组织病理学研究。结果显示,gr1在脾脏、鳃、头肾等组织中有较高的表达量,gr2则在肝、垂体、肠等组织中具有较高的表达丰度。应激恢复过程中,下丘脑gr1表达量存在波动,gr2在0 h表达显著上调,gr1/gr2值逐渐增大;垂体中gr1和gr2均呈现先升后降的趋势,gr1/gr2值在2 h达到峰值水平;头肾中gr1表达量有波动,而gr2在0 h和2 h的表达量显著低于其他检测点的表达量,gr1/gr2值逐渐减小;肝脏中gr1的表达量在0 h和2 h的表达量显著高于其他检测点,gr2表达有上下波动的现象,gr1/gr2值有减小的趋势,而pepck表达则出现了显著上调,在2 h达到峰值;皮肤中gr1先升高后降低,gr2在0 h和2 h的表达量显著低于其他检测点,gr1/gr2值在2～8 h维持在较大值,而occ表达呈现先增加后降低的变化趋势;鳃中gr1与gr2均在2 h时发生了显著上调,gr1/gr2值在2～8 h维持在较大值,occ表达峰值出现在2 h。组织学研究显示鳃丝有增生现象,肾间组织中类淋巴细胞增多,其他所检视组织无明显病理改变。应激反应过程中gr在不同组织不同亚型间存在不同的表达变化,以及和不同组织器官中相关功能基因的相关性显示了GR在相关调控中的复杂性,而组织学研究结果则表明了应激反应存在诱发病理变化的风险。
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| 关 键 词： | 团头鲂 糖皮质激素受体 应激 皮质醇 闭合蛋白 |
| 收稿时间： | 2018/4/5 0:00:00 |
| 修稿时间： | 2018/7/6 0:00:00 |
Tissue distribution and expression analyses of Megalobrama amblycephala gr in stress |
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| CHEN Kai,XI Bingwen,TENG Tao,QIN Ting,PAN Liangkun and XIE Jun.Tissue distribution and expression analyses of Megalobrama amblycephala gr in stress[J].Journal of Fisheries of China,2019,43(4):719-730. |
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| Authors: | CHEN Kai XI Bingwen TENG Tao QIN Ting PAN Liangkun and XIE Jun |
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| Institution: | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences, Wuxi 214081, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences, Wuxi 214081, China;Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, China,Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences, Wuxi 214081, China,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences, Wuxi 214081, China and Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences, Wuxi 214081, China |
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| Abstract: | In order to understand the biological function of glucocorticoid receptors (GRs) of Megalobrama amblycephala, we explored the expression characteristics of grs in different tissues and the expression changes of grs and some function genes in the recovery process after stress. Results show that the gr1 was highly expressed in gill, spleen and head kidney, while gr2 was highly expressed in pituitary, liver, intestinal, and so on. During the recovery process, on HPI axis, the expression of gr1 had fluctuations, gr2 significantly up-regulated at the 0 h, and the value of gr1/gr2 was gradually increasing in hypothalamus. The expression of gr1 and gr2 all rose first and then decreased, gr1/gr2 value reached the peak at 2 h in pituitary. The expression of gr1 had fluctuations, whereas gr2 had a low expression at 0 h and 2 h compared with the other time points, gr1/gr2 value gradually decreased in head kidney. In liver, gr1 rose in the early time (0 h and 2 h), and gr2 presented a fluctuating situation, and there was a tendency to reduce the the value of gr1/gr2. At the similar time point the expression of pepck peaked. In gills gr1 rose first, and then decreased, while the expression of gr2 was subdued in the early time (0 h and 2 h), gr1/gr2 value maintained at a large value during 2-8 h, while the occ expression peaked at 2 h. In skin gr1 and gr2 rose significantly in 2 h, gr1/gr2 in 2-8 h also maintained at a large value, and the occ expression rose first and then decreased. Histology research shows that gill filament was hyperplasia, lymphocyte-like cells increased kidney tissues, while other organizations examined had no obvious pathological changes. All the results indicate that GRs have a complex regulatory mechanism in the stress, and the histological analysis shows that the risks of pathological changes come from the stress. |
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| Keywords: | Megalobrama amblycephala glucocorticoid receptor stress cortisol occludin |
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