| 生物质炭与噬菌体联用阻控与灭活土壤-生菜体系中抗生素抗性致病细菌 |
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| 引用本文： | 赵远超,叶 茂,孙明明,张忠云,黄 丹,朱国繁,郑晓璇,晁会珍,冯彦房,武 俊,胡锋,蒋 新.生物质炭与噬菌体联用阻控与灭活土壤-生菜体系中抗生素抗性致病细菌[J].土壤,2019,51(5):942-948. |
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| 作者姓名： | 赵远超 叶 茂 孙明明 张忠云 黄 丹 朱国繁 郑晓璇 晁会珍 冯彦房 武 俊 胡锋 蒋 新 |
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| 作者单位： | 南京农业大学资源与环境科学学院,中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所),南京农业大学资源与环境科学学院,中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所),中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所),中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所),南京农业大学资源与环境科学学院,南京农业大学资源与环境科学学院,江苏省农业科学院农业资源与环境研究所,南京农业大学资源与环境科学学院,南京农业大学资源与环境科学学院,中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所) |
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| 基金项目： | 国家重点研发计划项目(2018FYC1803100)、2017年度江苏省环保科研重点项目(2017005)、2017年江苏省农业科技自主创新资金项目(CX(17)3047)、国家自然科学基金面上项目(41771350)和中国科学院青年创新促进会项目(2018350)资助。 |
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| 摘 要： | 农田土壤–蔬菜体系中残留和滋生的多种抗生素抗性致病细菌已对人体健康和生态环境安全造成较严重的隐患,因此开展针对性的风险管控技术研究十分迫切。生物质炭阻控与农业噬菌体疗法联用靶向灭活土壤–蔬菜体系中抗生素抗性致病细菌,为解决此类污染土壤问题提供了全新途径。本研究以自主制备的抗生素抗性致病细菌(携带四环素抗性基因tet W的大肠杆菌K12,携带氯霉素抗性基因amp C的铜绿假单胞菌PAO1)污染农田土壤为盆栽用土,开展生菜土培试验60d。设置单独或同时添加生物质炭和接种广宿主型噬菌体(YSZ 5K)的不同处理,以土壤–生菜体系中K12、PAO1数量变化及tet W、amp C丰度消减程度表征联合修复的效果。结果表明,针对土壤–生菜体系中残留K12、PAO1和tet W、amp C消减程度变化,判断不同处理效果,依次为:BP(生物质炭与噬菌体联用) B(单独施用生物质炭)P(单独接种噬菌体)CK(对照),其中BP处理条件下,K12与PAO1在土壤和生菜叶片中数量较之对照处理下降了2.1～3.1个数量级,tet W和amp C丰度较之对照处理下降了2.2～3.3个数量级。此外,在BP处理条件下,生菜收获后,土壤微生物群落结构与功能多样性和稳定性指数也得到显著提升,证明该联合治理方式是一种较为环境友好的修复技术。本研究结果可为降低土壤–蔬菜体系中抗性致病细菌的残留风险提供科学的理论依据和有效的管控技术。
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| 关 键 词： | 土壤-蔬菜体系 生物质炭 农业噬菌体疗法 抗生素抗性致病细菌 |
| 收稿时间： | 2018/3/26 0:00:00 |
| 修稿时间： | 2018/5/23 0:00:00 |
Combined Biochar and Bacteriophage to Control and Inactivate Antibiotic Resistance Pathogenic Bacteria in Soil-Lettuce System |
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| ZHAO Yuanchao,YE Mao,SUN Mingming,ZHANG Zhongyun,HUANG Dan,ZHU Guofan,ZHENG Xiaoxuan,CHAO Huizhen,FENG Yanfang,WU Jun,HU Feng and JIANG Xin.Combined Biochar and Bacteriophage to Control and Inactivate Antibiotic Resistance Pathogenic Bacteria in Soil-Lettuce System[J].Soils,2019,51(5):942-948. |
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| Authors: | ZHAO Yuanchao YE Mao SUN Mingming ZHANG Zhongyun HUANG Dan ZHU Guofan ZHENG Xiaoxuan CHAO Huizhen FENG Yanfang WU Jun HU Feng and JIANG Xin |
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| Institution: | College of Resources and Environmental Sciences, Nanjing Agricultural University,Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences,College of Resources and Environmental Sciences, Nanjing Agricultural University,Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences,Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences,Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences,College of Resources and Environmental Sciences, Nanjing Agricultural University,College of Resources and Environmental Sciences, Nanjing Agricultural University,Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences,College of Resources and Environmental Sciences, Nanjing Agricultural University,College of Resources and Environmental Sciences, Nanjing Agricultural University and Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences |
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| Abstract: | Ubiquitous existence of antibiotic resistant pathogens (ARP) in the agricultural land and vegetation system has posed great threat against public health and environmental safety, making it essential to develop targeted controlling technologies. In this work, biochar and bacteriophage combined technology was investigated for its effectiveness in targeted inactivating the ARP in the soil-lettuce system. Biochar and polyvalent phage YSZ 5K were applied to the ARP (tetracycline resistant Escherichia coli K12 and chloramphenicol resistant Pseudomonas aeruginosa PAO1) contaminated soil. The residual K12 and PAO1 counts and antibiotic resistance genes (tetW and ampC) abundances were determined after 60 days of incubation. The results showed that the combined technology was proved most effective, followed by sole application of biochar, sole inoculation of YSZ 5K, and the control. For the combined treatment, the counts of K12 / PAO1 decreased by 2.1-3.1 magnitudes and the abundances oftetW/ampC decreased by 2.2-3.3 magnitudes in the soil and lettuce leaves, respectively. In addition, the structural and functioning diversity of soil microbial community was improved significantly after the combined treatment. The results obtained here demonstrate the combined application of biochar and polyvalent phage YSZ 5K an environmentally-friendly technology that could effectively decrease the ARP in the soil-lettuce system. |
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| Keywords: | Soil-vegetable system Biochar Agricultural phage therapy Antibiotic resistant pathogenic bacteria |
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