| 柴胡总皂苷调控细胞色素P450酶发挥类似柴胡疏肝散抗抑郁作用研究 |
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| 作者姓名： | 贺泽慧 范荣 张春虎 唐涛 崔寒尽 刘胥 梁清华 王杨 |
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| 作者单位： | 中南大学湘雅医院中西医结合科, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008;中南大学湘雅医院中西医结合科全国名老中医梁清华传承工作室, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008;中南大学湘雅医院中西医结合科全国名老中医梁清华传承工作室, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008;中南大学湘雅医院中西医结合科全国名老中医梁清华传承工作室, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008;中南大学湘雅医院中西医结合科全国名老中医梁清华传承工作室, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008;中南大学湘雅医院中西医结合科全国名老中医梁清华传承工作室, 湖南 长沙 410008,中南大学湘雅医院中西医结合科, 湖南 长沙 410008;中南大学湘雅医院中西医结合科全国名老中医梁清华传承工作室, 湖南 长沙 410008 |
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| 基金项目： | 国家自然科学基金（81403259，81673951）。 |
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| 摘 要： | 目的 观察柴胡总皂苷（total saponins，TS）对肝气郁结证（liver-Qi stagnation，LQS）和肝郁脾虚证（liver stagnation and spleen deficiency，LSSD）小鼠抑郁行为的改善作用，并探讨其抗抑郁作用与CYP3A4的关系。方法 构建LQS、LSSD模型，TS干预14 d后，通过体质量测定、悬尾实验和强迫游泳实验评估小鼠抑郁样行为，并采用蛋白印迹法和逆转录定量聚合酶链式反应（polymerase chain reaction，PCR）法检测CYP3A4蛋白和mRNA的表达变化。结果 造模完成后，与空白组相比，模型组的体质量明显减轻，悬尾不动时间和强迫游泳不动时间明显增加（P<0.01）。TS干预14 d后，LQS模型小鼠悬尾不动时间和强迫游泳不动时间明显缩短（P<0.01），LSSD模型小鼠悬尾不动时间和强迫游泳不动时间无明显变化（P>0.05）。TS可下调LSD抑郁症模型小鼠CYP3A4蛋白的表达（P<0.01），而对其mRNA的表达影响无显著性差异（P>0.05）。结论 TS可改善LSD模型小鼠的抑郁样行为，其作用机制可能与下调CYP3A4蛋白的表达有关。
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| 关 键 词： | 柴胡总皂苷 抑郁症 肝气郁结 肝郁脾虚 CYP3A4 |
| 收稿时间： | 2019/1/4 0:00:00 |
Research on Total Saponins of Radix Bupleuri Regulating Cytochrome P450 Similar to Chaihu Shugan Powder Exerting Antidepressant Effects |
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| Authors: | HE Zehui FAN Rong ZHANG Chunhu TANG Tao CUI Hanjin LIU Xu LIANG Qinghua and WANG Yang |
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| Institution: | Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China,Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China;National Famous TCM Doctor Liang Qinghua''s Inheritance Studio, Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China,Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China;National Famous TCM Doctor Liang Qinghua''s Inheritance Studio, Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China,Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China;National Famous TCM Doctor Liang Qinghua''s Inheritance Studio, Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China,Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China;National Famous TCM Doctor Liang Qinghua''s Inheritance Studio, Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China,Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China,Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China;National Famous TCM Doctor Liang Qinghua''s Inheritance Studio, Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China and Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China;National Famous TCM Doctor Liang Qinghua''s Inheritance Studio, Department of Integrated Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China |
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| Abstract: | Objective To observe the anti-depressant effects of total saponins (TS) of Radix Bupleuri on mice with liver Qi stagnation (LQS) syndrome and liver stagnation and spleen deficiency (LSSD) syndrome, and to explore the relationship between the effects and CYP3A4. Methods LQS and LSSD models were established by binding and isolation. Depressive behaviors were evaluated via body mass measurement, tail suspension test and forced swimming test after 2 weeks of TS treatment. Expression changes of CYP3A4 protein and mRNA were determined by Western blot and RT-qPCR. Results After the establishment of models, compared with the blank group, the body mass in the model groups was significantly decreased; and the immobility time of tail suspension and forced swimming was significantly increased (P<0.01). After 2-week treatment with TS, the immobility time of LQS mice in tail suspension test and forced swimming test was significantly decreased (P<0.01), while that of LSSD mice was not obviously changed (P>0.05). The expression of CYP3A4 protein in LSSD mice could be decreased by TS (P<0.01), while there was no significant difference in its effect on the expression of mRNA (P>0.05). Conclusion TS can improve the depressive behaviors on LQS mice. The mechanism may be associated with the down-regulation on CYP3A4 protein expression. |
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| Keywords: | total saponins of Radix Bupleuri depression liver Qi stagnation liver stagnation and spleen deficiency CYP3A4 |
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