| 两种弧菌感染大黄鱼免疫相关基因的SNP位点分析 |
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| 引用本文： | 孙明洁,张娜,徐善良,鲍宝龙,龚小玲.两种弧菌感染大黄鱼免疫相关基因的SNP位点分析[J].上海海洋大学学报,2019,28(5):772-781. |
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| 作者姓名： | 孙明洁 张娜 徐善良 鲍宝龙 龚小玲 |
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| 作者单位： | 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306,宁波大学 应用海洋生物技术教育部重点实验室, 浙江 宁波 315211,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306 |
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| 基金项目： | 国家自然科学基金（31872546） |
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| 摘 要： | 为了探究大黄鱼(Larimichthys crocea)免疫相关基因的SNP与弧菌抗性关系,分别利用鳗弧菌(Vibrio anguillarum)和副溶血弧菌(Vibrio parahaemolyticus)人工感染大黄鱼。对感染前后抗感群体的转录组进行高通量测序、筛选并分析其抗病差异:(1)筛选氨基酸的非同义突变SNP位点在抗鳗弧菌组有17个,而抗副溶血弧菌组的有28个;(2)一代测序验证结果发现,染色体NW_011323507.1上白细胞介素6受体基因(IL-6R)第91 196位碱基G突变为C,导致缬氨酸突变为亮氨酸,该位点G/C在抗鳗弧菌组、对照组样本之间突变基因型CC频率分别为12.5%和0,呈显著性差异(P0.05);(3)补体C1q/肿瘤坏死因子相关蛋白9(CTRP9)基因在染色体NW_011323975.1上的35 665位碱基突变(A-G),在副溶血弧菌抗感易感群体中突变位点基因型GG频率分别为37.5%和0,呈极显著性差异(P0.01)。结果表明,IL-6R-91196-G/C位点突变与大黄鱼抗鳗弧菌有关联,CTRP9-35665-A/G位点突变与大黄鱼抗副溶血弧菌有关联,这为大黄鱼抗弧菌群体的选育提供了理论依据。
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| 关 键 词： | 鳗弧菌 副溶血弧菌 大黄鱼 转录组 免疫基因 SNP |
| 收稿时间： | 2019/4/29 0:00:00 |
| 修稿时间： | 2019/8/31 0:00:00 |
Analysis of SNP loci in immune-related genes of two species of Vibrio infecting large yellow croaker (Larimichthys crocea) |
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| SUN Mingjie,ZHANG N,XU Shanliang,BAO Baolong and GONG Xiaoling.Analysis of SNP loci in immune-related genes of two species of Vibrio infecting large yellow croaker (Larimichthys crocea)[J].Journal of Shanghai Ocean University,2019,28(5):772-781. |
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| Authors: | SUN Mingjie ZHANG N XU Shanliang BAO Baolong and GONG Xiaoling |
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| Institution: | The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, Zhejiang, China,The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China and The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to investigate the relationship between SNP and Vibrio resistance in immune-related genes of large yellow croaker (Larimichthys crocea), Vibrio anguillarum and Vibrio parahaemolyticus were used to artificially infect large yellow croaker. High-throughput sequencing, screening and analysis of disease-resistant groups before and after infection:(1)17 non-synonymous mutation sites of amino acid were screened from V. anguillarum resistance group.A total of 28 SNPs were screened from the V. parahaemolyticus resistance group.(2)The first-generation sequencing confirmed that the SNP loci was located on the 91196th of chromosome NW_011323507.1 that interleukin-6 receptor gene (IL-6R) was from G changed to C, the proline was mutated to leucine, and the genotype CC frequencies of this site in the V. anguillarum resistance and the control groups were 12.5% and 0, which showed significant difference (P<0.05).(3)And the 35665 base mutation (A-G) of the complement C1q/TNF-related protein 9 (CTRP9) gene on chromosome NW_011323975.1 showed a significant difference in the V. parahaemolyticus susceptibility/resistance groups (P<0.01), the genotype GG frequencies were 37.5% and 0. The results showed that IL-6R-91196-G/C site mutation was associated with V. anguillarum resistance group, and CTRP9-35665-A/G site mutation was associated with large yellow croaker against V. parahaemolyticus.It provides theoretical support for the selection and breeding of large yellow croaker against Vibrio population. |
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