| 云南玉溪地区烟田根结线虫的SCAR鉴定、分布及其生物防治研究 |
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| 引用本文： | 宋莉,周培培,李阳,张立猛,赵进龙,张翠萍,罗秀莲,纳红艳,刘芳,谷星慧,阮维斌.云南玉溪地区烟田根结线虫的SCAR鉴定、分布及其生物防治研究[J].农业环境科学学报,2019,36(4):546-552. |
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| 作者姓名： | 宋莉 周培培 李阳 张立猛 赵进龙 张翠萍 罗秀莲 纳红艳 刘芳 谷星慧 阮维斌 |
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| 作者单位： | 南开大学生命科学学院, 天津 300071,南开大学生命科学学院, 天津 300071,南开大学生命科学学院, 天津 300071,云南省烟草公司玉溪市公司, 云南 玉溪 653100;烟草行业病虫害生物防治工程研究中心, 云南 玉溪 653100,云南省烟草公司玉溪市公司, 云南 玉溪 653100;烟草行业病虫害生物防治工程研究中心, 云南 玉溪 653100,云南省烟草公司玉溪市公司, 云南 玉溪 653100,云南省烟草公司玉溪市公司, 云南 玉溪 653100,云南省烟草公司玉溪市公司, 云南 玉溪 653100,云南省烟草公司玉溪市公司, 云南 玉溪 653100,云南省烟草公司玉溪市公司, 云南 玉溪 653100;烟草行业病虫害生物防治工程研究中心, 云南 玉溪 653100,南开大学生命科学学院, 天津 300071 |
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| 基金项目： | 中国烟草总公司云南省公司科技计划项目（2017YN15，2014YN21） |
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| 摘 要： | 云南玉溪地区是我国的烟草主产区，根结线虫病是烟草生产中的重要地下病害，危害烟草植株生长，降低产量。本研究运用SCAR-PCR方法对云南玉溪地区根结线虫进行分子鉴定，并分析不同根结线虫种类在各县区的分布情况；同时在澄江、易门两县开展利用昆虫病原线虫对根结线虫的田间防治试验。结果表明，玉溪地区主要的优势种群为花生根结线虫Meloidogyne arenaria，在所有区县均有分布，爪哇根结线虫M.javanica为次要种群，南方根结线虫M.incognita属于零星分布；田间施加昆虫病原线虫对烟草根结线虫病具有显著的抑制作用。研究结果可为烟草根结线虫病的绿色防治提供数据支持。
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| 关 键 词： | 昆虫病原线虫，生物防治，根结线虫，玉溪，烟草 |
| 收稿时间： | 2018/8/30 0:00:00 |
Distribution of Meloidogyne spp. in tobacco field of Yuxi, Yunnan Province and biological control against Meloidogyne spp. |
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| SONG Li,ZHOU Pei-pei,LI Yang,ZHANG Li-meng,ZHAO Jin-long,ZHANG Cui-ping,LUO Xiu-lian,NA Hong-yan,LIU Fang,GU Xing-hui and RUAN Wei-bin.Distribution of Meloidogyne spp. in tobacco field of Yuxi, Yunnan Province and biological control against Meloidogyne spp.[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2019,36(4):546-552. |
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| Authors: | SONG Li ZHOU Pei-pei LI Yang ZHANG Li-meng ZHAO Jin-long ZHANG Cui-ping LUO Xiu-lian NA Hong-yan LIU Fang GU Xing-hui and RUAN Wei-bin |
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| Institution: | College of Life Sciences, Nankai University, Tianjin 300071, China,College of Life Sciences, Nankai University, Tianjin 300071, China,College of Life Sciences, Nankai University, Tianjin 300071, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China;Application Technology of Biological Control for Tobacco Diseases and Insect Pests Engineering Research Center of China Tobacco, Yuxi 653100, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China;Application Technology of Biological Control for Tobacco Diseases and Insect Pests Engineering Research Center of China Tobacco, Yuxi 653100, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China,Yuxi Company of Yunnan Provincial Tobacco Company, Yuxi 653100, China;Application Technology of Biological Control for Tobacco Diseases and Insect Pests Engineering Research Center of China Tobacco, Yuxi 653100, China and College of Life Sciences, Nankai University, Tianjin 300071, China |
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| Abstract: | Yuxi is the main tobacco producing area in China. Root-knot nematode disease is the important underground diseases in tobacco production, which has endangered tobacco plant growth and reduced tobacco yield. In this study, the SCAR-PCR method was used to identify the root-knot nematodes in Yuxi, Yunnan Province, and the distribution of different root-knot nematodes in each county was analyzed. The field experiment was performed in Chengjiang and Yimen Counties in Yuxi. The results showed that Meloidogyne arenaria was the dominant species in Yuxi region, which was distributed in all counties. M.javanica was the minor species, and M.incognita was rare in Yuxi. And the application of entomopathogenic nematodes in the field could significantly inhibit the occurrence of root-knot nematodes in tobacco. The results above could provide data support for the green control of tobacco root-knot nematode disease. |
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| Keywords: | entomopathogenic nematode biocontrol root-knot nematodes Yuxi tobacco |
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