| 电针对PCOS模型大鼠性激素及卵巢组织TSP-1表达的影响 |
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| 作者姓名： | 余小乔 李云君 |
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| 作者单位： | 湖北中医药大学, 湖北 武汉 430061,湖北省中医院妇产科, 湖北 武汉 430061 |
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| 基金项目： | 中科院中基所自主项目（YZ-1741）；湖北省中医药中西医结合科研项目（鄂卫生计生通[2017]20号） |
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| 摘 要： | 目的 探讨电针对多囊卵巢综合征（polycystic ovary syndrome，PCOS）模型大鼠的治疗作用，以及对卵巢组织中血小板反应蛋白-1（thrombospondin-1，TSP-1）表达的影响。方法 将32只雌性SD大鼠随机分为空白组10只、模型组12只、电针组10只，采用1 mg/kg来曲唑加l%羧甲基纤维素灌胃建立PCOS大鼠模型。治疗后检测大鼠血清睾酮（testosterone，T）、黄体生成素（luteinizing hormone，LH）、卵泡刺激素（follicle-stimulating hormone，FSH）水平，HE染色观察卵巢组织病理结构改变，免疫组化法检测卵巢组织中TSP-1的表达。结果 治疗后，电针组大鼠体质量增量和卵巢相对质量较模型组降低（P<0.01），血清T、LH水平及LH/FSH均较模型组降低（P<0.01），卵巢切片可见小囊状扩张卵泡较模型组减少，颗粒细胞层增厚；TSP-1表达于卵巢组织颗粒细胞和卵泡膜细胞的胞浆，模型组TSP-1表达量较低空白组降低（P<0.01），电针组TSP-1表达较模型组升高（P<0.05）。结论 电针治疗可改善PCOS大鼠性激素紊乱状态，提高卵巢组织中TSP-1的表达，通过调节血管抑制因子、减少卵巢组织中血管新生、改善微循环以达到治疗PCOS的目的。
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| 关 键 词： | 多囊卵巢综合征 电针 性激素 血小板反应蛋白-1 |
| 收稿时间： | 2018/9/6 0:00:00 |
Effects of Electroacupuncture on Sex Hormone and Expression of TSP-1 in Ovarian Tissue of PCOS Rats |
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| Authors: | YU Xiaoqiao and LI Yunjun |
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| Institution: | Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China and Obstertrics and Gynecology Department, Chinese Medicine Hospital of Hubei, Wuhan, Hubei 430061, China |
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| Abstract: | Objective To observe the therapeutic effects of electroacupuncture (EA) on polycystic ovary syndrome (PCOS) model rats and its effects on the expression of thrombospondin-1 (TSP-1) in ovarian tissues. Methods A total of 32 female SD rats were randomly divided into the blank group (n=10), the model group (n=12), and the EA group (n=10). PCOS rat model was established by intragastric administration of 1 mg/kg letrozole dissolved in 1% carboxymethylcellulose (CMC). After treatment, serum levels of testosterone (T), luteinizing hormone (LH), and follicle stimulating hormone (FSH) were measured by enzyme-linked immunosorbent assay. The pathological changes of the ovarian tissue were observed by HE staining. The expression of TSP-1 in ovarian tissues was detected by immunohistochemistry. Results Compared with the model group, the body weight gain and the relative ovarian mass of the EA group were decreased when compared with the model group (P<0.01), and the serum T, LH levels and LH/FSH were decreased when compared with the model group (P<0.01). The HE staining of the ovarian tissue showed decreased cystic expansion follicles, and the granular cell layer was obviously thicker. TSP-1 was expressed in the cytoplasm of ovarian tissue granulosa cells and ovarian membrane cells. The expression of TSP-1 was decreased in the model group than in the blank group (P<0.01). After treatment, the expression of TSP-1 in the EA group was increased when compared with model group (P<0.05). Conclusion Electroacupuncture can improve the state of sex hormone disorder in PCOS rats, increase the expression of TSP-1, reduce the angiogenesis in ovarian tissue by regulating angiogenesis inhibitors, and improve microcirculation to achieve the purpose of treating PCOS. |
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| Keywords: | polycystic ovary syndrome electroacupuncture sex hormone thrombospondin-1 |
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