Effect of freezing bull semen in two non‐egg yolk extenders on post‐thaw sperm quality |
| |
| Authors: | IB Lima‐Verde A Johannisson T Ntallaris E Al‐Essawe Z Al‐Kass T Nongbua F Dórea N Lundeheim K Kupisiewicz A Edman JM Morrell |
| |
| Institution: | 1. Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;2. University Tiradentes, Technology and Research Institute, Aracaju‐SE, Brazil;3. Clinical Reproductive Physiology, Al‐Nahrain University – High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Baghdad, Iraq;4. Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq;5. Clinical Sciences, Mahasarakham University, Mahasarakham, Thailand;6. National Veterinary Institute, Uppsala, Sweden;7. Department of Animal Breeding and Genetics, SLU, Uppsala, Sweden;8. Viking Genetics, Randers S?, Denmark;9. Viking Genetics, ?rnsro, Skara, Sweden |
| |
| Abstract: | Traditionally, extenders for bull semen included egg yolk or milk, but recently there has been a move to avoid material of animal origin. The aim of this study was to evaluate the effects of two commercial extenders (based on soya lecithin and liposomes) on bull sperm quality after cryopreservation. Post‐thaw sperm quality was evaluated by computer‐assisted sperm analysis and flow cytometric assessment of membrane integrity, chromatin integrity, mitochondrial membrane potential, production of reactive oxygen species and tyrosine phosphorylation. Furthermore, an artificial insemination (AI) trial was conducted, and 56‐day non‐return rates were evaluated. Semen frozen in the liposome‐based extender showed similar membrane integrity and higher mitochondrial membrane potential compared to those in the soya lecithin‐based extender. Chromatin integrity and production of live H2O2+ reactive oxygen species were similar in both extenders. Less superoxide was produced in the samples extended with liposome‐based extender, with or without menadione stimulation. Chromatin integrity and tyrosine phosphorylation were not affected by either type of extender. No differences in 56‐day non‐return rate between extenders containing soya lecithin and liposomes were observed in the AI trial (66% ± 0.8 and 65% ± 0.8, respectively). In conclusion, the sperm quality of bull semen frozen in the two extenders that do not contain material of animal origin was similar, although the semen frozen in the liposome‐based extender had higher mitochondrial membrane potential. Either extender could be used in situations where extenders containing material of animal origin are to be avoided. |
| |
| Keywords: | AI trial AndroMed® mitochondrial membrane potential OPTIXcell® reactive oxygen species production tyrosine phosphorylation |
|
|
